京尼平交联脱细胞猪真皮基质材料的研究  

Study on porcine acellular dermal matrix crosslinked by genipin

作　　者：但年华[1,2] 但卫华[1,2] 林海[1] 关林波[3] 肖世维[1] 

机构地区：[1]四川大学制革清洁技术国家工程实验室,四川成都610065 [2]四川大学生物医学工程技术研究中心,四川成都610065 [3]四川大学华西第二医院西部妇幼医学研究院,四川成都610065

出　　处：《功能材料》2012年第21期2966-2969,共4页Journal of Functional Materials

基　　金：成都市科技计划资助项目(10GGYB345SW-023);四川大学青年教师科研启动基金资助项目(2011SCU11102)

摘　　要：分别采用0、2%、4%、8%、16%(质量分数)的京尼平(GP)交联脱细胞猪真皮基质(pADM),检测交联后材料(GP-pADM)的基本性能。研究结果表明,随着交联剂GP用量的增加,材料的收缩温度逐渐提高,抗张强度降低;电镜扫描图显示交联后材料的孔隙有缩小的趋势,并导致了吸附水率、吸湿率和溶胀率的逐渐下降;红外图谱表明交联后材料中含有共轭双键;细胞毒性试验显示,GP-pADM细胞毒性为0级,急性经口毒性实验未见明显急性毒性。Porcine acellular dermal matrix （pADM） was crosslinked by serial dosage 0, 2~, 4~, 6%, 8%, 16wt% of genipin （GP） respectively, and the properties of which were characterized with shrinkage temperature （Ts）, rate of hydroscopicity, adhered water, absorbtion water, FT-IR, and SEM before and after crosslinked. With the increase of GP concentration, Ts of GP-pADM was enhanced obviously, but tensile strength declined. Observed from the SEM images of the material surface, the holes of GP-pADM tended to shrink after crosslinked, which resulted in the fall of the rate of hydroscopicity, adhered water, absorbtion water. Conjugated bouble-bonds were discovered and formed a new absorption in 2358.48cm^-1 after crosslinking. Acute oral toxicity did not appear and cytotoxicity of extraction obtainted from them respectively didn＇t observe in L-929 fibroblasts cultivated experiment. It was concluded that genipin was a superior crosslink agent for pADM.

关 键 词：脱细胞猪真皮基质 京尼平 交联 

分 类 号：R318.08[医药卫生—生物医学工程]