体外法优化肉鸡日粮非淀粉多糖酶  

Study on Optimization of Non-Starch Polysaccharide Enzymes of Broiler Diets in Vitro

作　　者：何科林[1] 萨仁娜[1] 高杰[1] 李东卫[1] 庄晓峰[1] 张宏福[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所/动物营养学国家重点实验室,北京100193

出　　处：《中国农业科学》2012年第21期4457-4464,共8页Scientia Agricultura Sinica

基　　金：农业部现代农业产业技术体系建设专项资金(CARS-42);国家科技支撑计划项目(2012BDA39B01);国家公益性行业科研专项经费(201003011-2)

摘　　要：【目的】探讨非淀粉多糖酶对肉鸡玉米日粮和小麦日粮离体消化能的调控。【方法】首先采用单因素随机试验设计,测定非淀粉多糖酶与日粮离体消化能的剂量关系。5种非淀粉多糖(non-starch polysaccharides,NSP)酶分别为:木聚糖酶、β-葡聚糖酶、纤维素酶、果胶酶和β-甘露聚糖酶,在肉鸡玉米-豆粕和小麦-豆粕日粮中分别添加0、100、200、300、400、500、600、700、800和900μg.g-1,分析NSP酶改善日粮离体消化能的作用效果。然后采用二次回归旋转正交组合试验设计,筛选两种日粮中5种NSP酶的最佳酶谱。离体消化能的测定采用胃蛋白酶-胰液素两步酶水解法。【结果】5种非淀粉多糖酶对离体消化能的调控效应显示规律性的"S"型曲线,木聚糖酶对玉米-豆粕、小麦-豆粕日粮的离体消化能提升程度较高,分别提高0.24和0.40 MJ.kg-1。在本试验条件下,玉米-豆粕日粮的最佳酶谱为:木聚糖酶34 836.4 U.kg-1、β-葡聚糖酶6 762.0 U.kg-1、纤维素酶1 159.2U.kg-1、果胶酶872.6 U.kg-1和β-甘露聚糖酶24 535.9 U.kg-1;小麦-豆粕日粮的最佳酶谱为:木聚糖酶65 405.5U.kg-1、β-葡聚糖酶10 131.5 U.kg-1、纤维素酶980.0 U.kg-1、果胶酶501.6 U.kg-1和β-甘露聚糖酶5 141.4 U.kg-1。【结论】肉鸡玉米-豆粕日粮、小麦-豆粕日粮中5种NSP酶联合使用能够改善日粮的离体消化能。【Objective】The study was conducted to discuss the effects of non-starch polysaccharides （NSP） enzymes on the in vitro digestive energy （IVDE） of broiler diets of different contents of NSP.【Method】The first step was to investigate the dose-response of the NSP enzymes on the IVDE in diets using one-way randomized experimental design. Five NSP enzymes including xylanase, β-glucanase, cellulose, pectinase, and β-mannanase were added into corn-soybean diet and wheat-soybean diet, from 0 to 900 μg？g-1. The second step was to screen the optimum enzyme combinations using quadratic regress-orthogonal rotary design. The method of testing IVDE was a two-stage enzymic hydrolysis with pepsin-pancreatin in vitro.【Result】The IVDE of broiler diet regulating by NSP enzyme appeared S state diagram. The xylanase had more promotion effects than other NSP enzymes. It increased IVDE by 0.24 MJ？kg-1 and 0.40 MJ？kg-1 in corn-soybean diet and wheat-soybean diet, respectively. In corn-soybean diet, the optimum enzyme combinations were xylanase 34 836.4 U·kg^-1, β-glucanase 6 762.0 U·kg^-1, cellulose 1 159.2 U·kg^-1, pectase 872.6 U·kg^-1, and β-mannanase 24 535.9 U·kg^-1. In wheat-soybean diet, the optimum enzyme combinations were xylanase 65 405.5 U·kg^-1, β-glucanase 10 131.5 U·kg^-1, cellulose 980.0 U·kg^-1, pectase 501.6 U·kg^-1, and β-mannanase 5 141.4 U·kg^-1. 【Conclusion】The findings showed that using five kinds of NSP enzymes in corn-soybean and wheat-soybean diets of broiler could improve the in vitro digestive energy.

关 键 词：非淀粉多糖酶 离体消化能 酶谱 肉仔鸡 体外法 

分 类 号：S831.5[农业科学—畜牧学]