铜绿假单胞菌PAO1降解菲、萘的特性及产物分析  

The characteristics and product analysis of phenanthrene and naphthalene degradation by Pseudomonas aeruginosa

作　　者：郜香黎[1] 李兆格[1] 张丹[1] 赵鑫[1] 李博[1] 阴灵芳[1] 陈林[1] 段康民[1] 沈立新[1] 

机构地区：[1]西北大学生命科学学院/西部资源与现代生物技术省部共建教育部重点实验室,陕西西安710069

出　　处：《西北大学学报（自然科学版）》2012年第5期777-782,共6页Journal of Northwest University（Natural Science Edition）

基　　金：国家自然科学基金资助项目(81171620);陕西省自然科学基金资助项目(2011JM 4013);陕西省教育厅基金资助项目(11JK0611);西北大学大学生校创基金项目资助

摘　　要：目的在前期发现实验室保存的铜绿假单胞菌PAO1具有较强的菲、萘降解能力基础上,对该菌株的菲、萘降解特性及代谢产物进行研究和分析。方法在MM基础培养基中通过改变各种因素来确定PAO1降解的最适条件,应用TLC和HPLC等方法确定了PAO1降解菲、萘过程中存在的重要代谢产物。结果 PAO1对菲、萘的最适降解温度为37℃,最适降解pH值为7.0,对菲、萘的降解属于好氧途径,添加表面活性剂或通过添加低抑制浓度抗生素而增加生物表面活性剂鼠李糖脂的产生都可增加菲、萘的降解,后者效果更好。初步推测PAO1的菲降解存在水杨酸途径和邻苯二甲酸途径;萘降解过程存在水杨酸途径,不存在龙胆酸途径。结论对后续降解途径的进一步清晰以及在此基础上降解酶的确定和工程菌株的构建奠定了基础。Aim Previous study has revealed that Pseudomonas aeruginosa PAO1 could degrade phenanthrene and naphthalene effectively. In this study, the culture conditions for degradation were optimized and the phenanthrene and naphthalene degradation pathways in PAO1 had been investigated. Methods The characteristics of PAHs deg- radation by PAO1 were determined by changing the growth conditions in MM medium. TLC and HPLC were used to analyse the degradation pathway and products. Results The optimized conditions for phenanthrene and naphtha- lene degradation by PAO1 were 37~C, pH 7.0 in aerobic environment. Presence of surfactant Tween - 80 at 0. 1 0. 2 g/L and subinhibitory concentrations of tetracycline and vancomycin could facilitate the degradation process. The antibiotic effect was possibly due to their effect on biosurfactant-rhamnolipid production. The production of rh- amnolipid was enhanced by subinhibitory concentrations of these two antibiotics. The phenanthrene degradation pathway in PAO1 involved both salicylic acid pathway and phthalic acid pathway while naphthalene degradation in- volved only the salicylic acid pathway. Conclusion The results expand the further study on the subsequent degra- dation pathway and build the foundation for the identification of bottleneck degrading enzymes and construction of engineering strains.

关 键 词：铜绿假单胞菌 多环芳烃 降解特性 降解途径 

分 类 号：Q939.99[生物学—微生物学]