花色苷对腺苷二磷酸激活血小板功能抑制作用  

Anthocyanins inhibit functions of human platelet induced by adenosine diphosphate

作　　者：石振银[1] 杨燕[1] 朱艳娜[1] 王冬亮[1] 吕小飞[1] 凌文华[1] 

机构地区：[1]中山大学公共卫生学院广东省营养膳食与健康重点实验室,广东广州510080

出　　处：《中国公共卫生》2012年第11期1452-1454,共3页Chinese Journal of Public Health

基　　金：国家自然科学基金(30972481)

摘　　要：目的探讨花色苷矢车菊-3-葡萄糖苷(Cy-3g)对健康人血小板活化和聚集的影响及其可能的机制。方法流式细胞仪测定全血中血小板活化膜糖蛋白IIbIIIa(GPIIbIIIa)和P-选择素(P-selectin)的阳性表达率;血小板聚集仪检测腺苷二磷酸(ADP)诱导的最大聚集率;荧光酶标仪分析Cy-3g对血小板胞浆钙离子水平的影响。结果 50μmol/L Cy-3g组GP IIb IIIa表达率[(73.92±14.02)%]与对照组[(93.36±2.02)%]比较,差异有统计学意义(P<0.05);P-选择素的表达率各组间差异无统计学意义(P>0.05);不同浓度的Cy-3g均在体外明显抑制富集血小板血浆的聚集(P<0.001);50μmol/L Cy-3g组可以明显抑制ADP诱导的血小板钙离子荧光强度值达(51.73±6.89)%(P<0.01)。结论花色苷Cy-3g可以抑制由ADP诱导的血小板活化GPIIbIIa的表达和血小板体外聚集,血小板胞浆钙离子浓度的降低可能是花色苷抑制作用的机制之一,提示Cy-3g对于预防心血管疾病具有积极作用。Objective To explore the effect of anthocyanin cyanidin-3-glucoside（Cy-3g）on human platelet activation,aggregation and its mechanism.Methods The expression of activiated GPIIbIIIa and P-selectin on platelet surface were examined with flow cytometry in vitro.Platelet aggregation induced by adenosine diphosphate（ADP） was measured with platelet aggregometer;calcium level in the platelet cytoplasma was analyzed with Fluo-3 AM staining.Results The expression of activated GPIIbIIIa was significantly decreased by 50 μmol/L Cy-3g（73.92%±14.02%） compared with the control buffer（93.36%±2.02%,P0.05）.The expression of P-selectin on platelet surface was not significantly different among the groups.Platelet aggregation of human platelet rich plasma（PRP）induced by ADP can be inhibited by Cy-3g at different doses（0.5 μmol/L,5 μmol/L,and 50 μmol/L）,which were all significantly lower than that of the control（P0.001）.The fluorescence intensity of calcium was decreased by 50 μmol/L Cy-3g（51.73±6.89%） in washed platelets（P0.01）.Conclusion Cy-3g could inhibit the expression of platelet GPIIbIIIa and aggregation induced by ADP in vitro.The decreased platelet cytoplasmic calcium may be one of the mechanism.The results suggest that Cy-3g may have a positive role in the prevention of cardiovascular diseases.

关 键 词：花色苷 血小板活化 血小板聚集 胞浆钙离子 腺苷二磷酸(ADP) 

分 类 号：R558[医药卫生—血液循环系统疾病]