柴胡皂苷d对阻塞性黄疸大鼠肝细胞游离钙及基质交联分子1的调控机制  

Modulatory mechanism of saikosaponin-d on intraceUular free calcium and stromal interaction mol-ecule 1 in the liver of rats with obstructive jaundice

作　　者：戴维[1] 陈念平[1] 陈赛红 包仕廷[1] 廖博懿[3] 刘刚[3] 

机构地区：[1]广东医学院附属医院肝胆外科,湛江524001 [2]门诊药房 [3]广东医学院2008硕士研究生

出　　处：《中华实验外科杂志》2012年第11期2122-2124,共3页Chinese Journal of Experimental Surgery

摘　　要：目的观察柴胡皂苷d（SSd）对阻塞性黄疸大鼠胆汁酸代谢、肝细胞游离钙及基质交联分子1（STIMl）基因表达的影响。方法将108只SD雄性大鼠随机分为假手术对照组、阻黄组、阳性药物对照组、高、中、低剂量SSd组，同时各组随机分7、14、21d3个时段，每时段每组6只，建模成功后，分别连续给药7、14、21d，于各时段末取肝组织和下腔静脉血标本，检测血清总胆汁酸（TBA）；应用流式细胞仪通过荧光指示剂Fluo-3／AM测定肝细胞内钙离子浓度；逆转录一聚合酶链反应（RT-PGR）检测STIMl的mRNA表达。结果阻黄模型建立7、14、21d后，阻黄组血清TBA逐步增加，肝细胞游离钙平均荧光强度（MFI）值逐步上升，STIMlmRNA表达逐步减弱；SSd治疗组7d末，与阻黄组比较，低、中、高剂量组TBA水平逐步下降，差异有统计学意义（P〈0．05）；肝细胞游离钙MFI值逐步下调，差异有统计学意义（P〈0．05）；STIMImRNA表达逐步增强，差异有统计学意义（P〈0．05）；14、21d末各检测指标变化趋势与7d末相同。结论SSd能降低血清TBA浓度，上调STIMlmRNA表达，抑制肝细胞钙超载，从而减轻阻塞性黄疸大鼠肝脏损害。Objective To observe the modulatory effects of saikosaponin-d （SSd） on the concen- tration of total bile acid （TBA）, intracellular free calcium, and the expression of stromal interaction mole- cule 1 （STIM1） in the liver of rats with obstructive jaundice. Methods 108 male SD rats were randomly divided into six groups, and each one was subdivided into three date-time subgroups （n = 6 each）. After models were established successfully, rats in each group were injected with corresponding drugs daily and killed at 7th, 14th, and 21st day. The concentration of TBA and free calcium in hepatocytes were meas- ured, and the expression of STIM1 mRNA in the liver was detected. Results At 7th, 14th, and 21st day, TBA level and the MFI of calcium in BDL group were progressively reduced, and the expression of STIM1 mRNA was progressively decreased. As compared with BDL group, TBA level and MFI of calcium in SSd groups was progressively decreased at 7th day （ P 〈 0. 01 ）, and the expression of STIM1 was pro- gressively increased （P 〈0. 01 ）. At 14th and 21st day, the changing trend of target index was identical. Conclusion SSd could decrease the concentration of TBA in plasma, enhance the expression of STIM1 and inhibit the calcium overload in hepatocytes.

关 键 词：柴胡皂苷D 阻塞性黄疸 基质交联分子1 钙库操纵性钙通道 

分 类 号：R285[医药卫生—中药学]