硝普钠对高胆固醇血症兔Oddi括约肌运动的影响  

Effects of sodium nitroprusside on movement of sphincter of Oddi in hypercholesterolemia rabbits

作　　者：谢斌[1] 田雨[1] 吴硕东[1] 国星奇[1] 

机构地区：[1]中国医科大学附属盛京医院胆道血管外科,沈阳110004

出　　处：《中华实验外科杂志》2012年第11期2128-2130,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30901439）

摘　　要：目的观察外源性一氧化氮（NO）供体硝普钠（SNP）对高胆固醇（Ch）血症兔Oddi括约肌（SO）运动的影响及对不同钾离子通道的作用。方法将24只新西兰大白兔随机分为对照组、4-氨基吡啶（4-AP）实验组及盐酸四乙胺（TEA）实验组，给予高Ch饮食饲喂8周，通过等长张力记录方法，观察SO肌环对钾离子通道阻滞剂4-AP和TEA的收缩反应，并观察比较SNP对各组SO肌环的舒张作用。结果SO肌环初始收缩平均波幅为（0．144±0．004）g，平均收缩频率为（10．8±1．1）7欠／min。累积浓度4-AP对SO肌环有收缩作用，收缩频率较对照组减低（P〈0．05），以16mmol／L为终质量浓度的4-AP组肌环对SNP舒张作用明显低于对照组（P〈0．05）。累积浓度TEA也可明显收缩S0肌环，收缩作用较对照组明显增强，收缩频率较对照组减低（P〈0．05）。以10mmol／L为终质量浓度的TEA组肌环对SNP舒张作用同样低于对照组（P〈0．05）。结论外源性NO供体SNP可能部分通过钾（KV）及大电导钙激活钾通道（BKCa）通道发挥舒张SO肌环的作用。Objective To study the-effects of nitric oxide-（NO） donor sodium nitroprusside （SNP） on the movement of sphincter of Oddi （SO） and different potassium channels in the hypercholester- olemia （CH） rabbits. Methods A total of 24 New Zealand rabbits were divided randomly into three groups ： control group （ n = 8 ）, experimental group of 4-aminopyridine （4-AP, n = 8 ） and experimental group of tetraethylammonium （TEA, n = 8）. The rabbits in each group were fed on high-cholesterol diet for 8 week. By using the method of the isometric tension recording, the contractile response of SO rings to 4-AP and TEA, and the relaxant effect of SNP on SO rings in all groups were measured. Results The ini- tial contraction of the average volatility was （0. 144 ±0. 004） , the average contraction frequency was （10. 8 ± 1.1 ）/min. SO rings showed the contractile response to the cumulation concentrations of 4-AP, and the contraction frequency was significantly reduced in 4-AP group as compared with the control group （P 〈0. 05）. With the final concentration of 16 mmol/L 4-AP, the relaxant effect of SNP on SO rings was significantly reduced as compared with control group （ P 〈 0. 05 ）. The accumulation concentrations of TEA could significantly shrink the SO muscle. The contraction was significantly increased, and the contraction frequency was significantly decreased in TEAigroup as compared with the control group （P 〈0. 05）. With the final concentration of 10 mmol/L TEA, relaxant effect of SNP on SO rings was also reduced as com- pared with the control group （ P 〈 0. 05 ）. COnclusion The NO donor SNP may play an important role in relaxation of the SO muscle through the KV and BKCa channels.

关 键 词：ODDI括约肌功能障碍 高胆固醇血症 肌张力 钾离子通道 一氧化氮 

分 类 号：R589.2[医药卫生—内分泌]