不同照射剂量下大鼠急性放射性肠炎模型的建立与比较  

Establishment and comparison of acute radiation enteritis models in rats with different irradiationdoses _

作　　者：洪智攀[1,2] 武寒飞[2] 赵克[1] 丁健华[1] 尹淑慧[1] 

机构地区：[1]解放军第二炮兵总医院肛肠外科全军肛肠病专病中心,北京100088 [2]辽宁医学院

出　　处：《中华实验外科杂志》2012年第11期2317-2320,共4页Chinese Journal of Experimental Surgery

基　　金：全军医学科研“十二五”计划资助项目（CWSllJ032）

摘　　要：目的建立不同照射剂量下大鼠放射性肠炎模型及比较其各自特点，探讨其发病机制。方法雄性SD大鼠90只，随机分为正常对照A组（18只）、小剂量5GyB组（24只）、中剂量10GyC组（24只）及高剂量15GyD组（24只），直线加速器对大鼠腹部一次性照射，在照射后2、4、7d，观察大鼠的体质量变化、死亡率、回盲部细菌移位率，光镜下观察小肠组织形态学变化，检测空肠一氧化氮（NO）的含量及采用酶联免疫吸附试验（ELESA）法检测空肠黏膜促炎细胞因子白细胞介素（IL）-18、IL-6、IL-8、肿瘤坏死因子（TNF）-α和抗炎细胞因子IL4、IL-10、IL-1受体拮抗剂（IL-lra）的蛋白含量。结果放射后第2天，放射组均出现不同程度的精神差、进食少、腹泻、黏液血便等症状，与A组比较，B组各指标均无统计学意义（P〉0．05），而C、D组差异有统计学意义（P〈0．05），出现放射性肠炎，且D组较C组改变更大（P〈0．05）；第4天放射组各症状均加重，且D组大鼠全部死亡，与A组比较，B、C、D组各指标差异有统计学意义（P〈0．05），均存在放射性肠炎，且程度B组〈C组〈D组（P〈0．05）；第7天，C组仍存在各症状但较前缓解，与A组比较，B组各指标差异无统计学意义（P〉0．05），C组差异有统计学意义（P〈0．05），C组与B组比较差异有统计学意义（P〈0．05）。结论本模型在不同的时间段均有各自典型的特点，适合放射性肠炎治疗研究的模型需要；放射性肠炎发病机制与NO及细胞因子变化存在密切的关系。Objective To establish and compare acute radiation enteritis models in rats with dif- ferent irradiation doses, and study the pathogenesis. Methods Ninety male SD rats were divided randomly into four groups： group A （control group：n = 18） , group B （low dose with 5 Gy：n =24） , group C （medi- um dose with 10 Gy：n =24）, group D （ high dose with 15 Gy：n =24）. After a single irradiation on rat＇ s abdomen using linear accelerator, weight, mortality and bacterial translocation of ileoceeum after irradiation at 2nd, 4th and 7th day were observed. Morphologic indexes Were measured by using light mieroseopy. Ni- tric oxide（NO） concentration of jejunum was measured. Protein contents of proinflammatory cytokines [ in- terleukin （IL）-1β, IL-6, IL-8, tumor necrosis factor （TNF）-α] and anti-inflammatory cytokines （IL-4, IL-10, IL-1ra） were examined by ELESA. Results At day 2, all irradiated groups had mental status with less eating, diarrhea and bloody stools with mucus. There were no significant differences in all indexes be- tween groups A and B （ P 〉 0. 05 ）. However there were significant differences between group C or group D and group A （ P 〈 0.05 ）. The changes were more severe in group D than in group C （ P 〈 0. 05 ）. At day 4, the symptoms of all irradiated groups were more severe and the rats in group D were all died. The inde- xes in groups B, C and D were significantly different from those in group A （ P 〈 0.05 ）. The severity of existed radioactive enteritis was group B 〈 group C 〈 group D（ P 〈0. 05 ）. At day 7, there still existed all symptoms in group C, but they were alleviated. There was no significant difference in all indexes be- tween group A and group B （ P 〉 0.05 ）, but there were significant difference between group A and group C （ P 〈 0. 05 ）, and between group C and group B （ P 〈 0. 05 ）. Conclusion In our radiation enteritis mod- els, there were different characters at different time Points after irradiat

关 键 词：放射性肠炎 细胞因子 一氧化氮 模型 动物 

分 类 号：R285.5[医药卫生—中药学]