Study on Outer Membrane Protein Patterns of Escherichia coli O38,O53 and O75 Isolated from Chickens  

鸡源大肠杆菌O38、O53及O75分离株外膜蛋白型的研究(英文)

作　　者：张艳英[1] 高桂生[1] 高光平[1] 史秋梅[1] 刘玉芹[1] 张海莹[1] 房海[1] 陈翠珍[1] 

机构地区：[1]河北科技师范学院,河北省预防兽医学重点实验室,河北昌黎066600

出　　处：《Agricultural Science & Technology》2012年第10期2070-2072,2122,共4页农业科学与技术（英文版）

基　　金：Supported by China Postdoctoral Science Foundation(20100470565);Science and Technology Support Program of Hebei Province(10960408D);Project of Science and technology Bureau of Shijiazhuang(1150093A);Science and Technology Development Project of Qinhuangdao City(201101A182)~~

摘　　要：[Objective] This study aimed to investigate the outer membrane protein （OMP） patterns of Escherichia coli 038, 053 and 075 isolates from chickens. [Method] Eight pathogenic E. coil isolates with various serotypes were used as experimental materials to extract OMP by using supersonic schizolysis method and Sarcosyl. After SDS-PAGE electrophoresis, OMP patterns of the extracted products were determined based on the OMP model diagram. [Result] OMP of eight E. coil isolates with three serotypes were divided into three patterns, to be specific, 2 075 isolates respectively belonged to OMP-I and OMP-II pattern, 1 053 isolate belonged to OMP-II pattern, and 5 038 isolates belonged to OMP-I and OMP-III pattern. [Conclusion] Experimental results showed that E. coli isolates with the same serotype may belong to completely different OMP patterns, while serologically unrelated isolates may belong to the same OMP pattern. OMP of E. coil isolates with the same serotype may generate genetic differentiation; in addition, OMP of E. coli isolates with different serotypes may have different genetic correlation.[目的]研究鸡源大肠杆菌血清型O38、O53及O75分离株的外膜蛋白型。[方法]以8株不同血清型鸡致病性大肠杆菌为试验菌株,采用超声波裂解和N-十二烷基肌胺酸钠对其进行外膜蛋白(OMP)的提取,提取产物经SDS-PAGE电泳检测后,根据OMP型模式图确定其OMP型。[结果]3个血清型的8株大肠杆菌外膜蛋白共分为3个型。其中2株O75血清型大肠杆菌的外膜蛋白分别为OMP-I型和OMP-II型,1株O53血清型大肠杆菌的外膜蛋白为OMP-II型,5株O38血清型大肠杆菌的外膜蛋白分为OMP-I型和OMP-III型。[结论]该研究结果表明,同一血清型的大肠杆菌菌株外膜蛋白可能属于完全不同的OMP型,血清学上毫不相关的分离株之间的外膜蛋白却可具有相同的OMP型。同一血清型的分离株的外膜蛋白之间可发生遗传分化,而不同血清型的分离株的外膜蛋白之间也可具有不同程度的遗传相关性。

关 键 词：Escherichia coli from chickens Outer membrane protein pattern SDS- PAGE 

分 类 号：S852.61[农业科学—基础兽医学]