二十碳五烯酸联合依托泊苷对人肺腺癌A-549细胞增殖和凋亡的影响  

Effects of eicosapentaenoic acid combined with etoposide on proliferation and apoptosis of human lung cancer cell line A-549

作　　者：马刘江[1] 安京华[1] 李星[2] 李香善[1] 石俊[1] 

机构地区：[1]吉林省延边大学附属医院ICU,延吉133000 [2]首都医科大学燕京医学院附属北京大兴医院急诊科,102600

出　　处：《中华临床营养杂志》2012年第5期306-310,共5页Chinese Journal of Clinical Nutrition

基　　金：国家自然科学基金资助项目（30560152）

摘　　要：目的研究二十碳五烯酸（EPA）联合依托泊苷对人肺腺癌A-549细胞株增殖与凋亡的影响。方法分别用终浓度为40μg/mlEPA、10pg／ml依托泊苷、40μg/mlEPA＋10μg／ml依托泊苷、20μg／ml依托泊苷、40μg／mlEPA＋20μg／na依托泊苷孵育A-549细胞，采用MTT法、膜联蛋白V-异硫氰酸荧光素／碘化丙锭双染法、吖啶橙／溴化乙锭双染法及流式细胞术研究细胞增殖抑制率、细胞形态学及凋亡情况。结果EPA联合依托泊苷对A-549细胞的增殖抑制率显著高于依托泊苷单独的作用（P均〈0．05）。细胞荧光染色显示：EPA联合依托泊苷组的凋亡细胞数量多于依托泊苷单独作用组。EPA联合依托泊苷组的s期及G2／M期细胞显著多于依托泊苷单独作用的细胞（P均〈0．05）。结论EPA可能具有增强依托泊苷抑制人肺腺癌A-549细胞增殖、促进A-549细胞凋亡的作用。Objective To study the effects of eicosapentaenoic acid （EPA） combined with etoposide on the proliferation and apoptosis of human lung cancer cell lines A-549 in vitro. Methods The A-549 cens were cultured, respectively, with culture fluids containing 40 μg/ml EPA, 10 μg/ml etopeside, 40 μg/ml EPA ＋ 10 μml etope- side, 20μg/ml etoposide, or 40μg/ml EPA ＋ 20 μml. The effects of EPA and/or etopeside on the proliferation of the cell line were detected by MTT assay. The morphological changes of the ceils were observed using annexin V-flu- orescein isothiocyanate/propidium iodine and acridine orange/ethidium bromide double staining under fluorescence microscope. The apoptosis and the changes of ceil cycle were detected by flow cytometry. Results The inhibition rates of EPA plus etoposide groups on the proliferation of human lung cancer cells A-549 were significantly higher than those in other etoposide groups （ all P 〈 0. 05）. Cell staining showed that the amount of apoptotic ceils in EPA plus etoposide groups was significantly larger than that in etoposide groups. The percentages of cens in S and G2/M phases of EPA plus etoposide groups were significantly higher than those of etoposide groups （ an P 〈 0. 05）. Con- clusion EPA may enhance the effect of etopeside on inhibiting proliferation and promoting apoptosis for human lung cancer ceils A-549.

关 键 词：二十碳五烯酸 依托泊苷 A-549细胞 凋亡 

分 类 号：R734.2[医药卫生—肿瘤]