丝瓜种质资源亲缘关系的SRAP分析  

Genetic Diversity Analysis of Germplasm Resources of Towel Gourd Based on SRAP Markers

作　　者：崔竣杰[1] 宋建文[1] 汪国平[1] 林明宝[1] 李连芳 胡开林[1] 

机构地区：[1]华南农业大学园艺学院,广州510642 [2]广州市农业科学院,广州510308

出　　处：《植物遗传资源学报》2012年第6期1061-1066,共6页Journal of Plant Genetic Resources

基　　金：广州市科技计划项目(2010Z1-E381);广东现代农业产业技术体系建设专项资金

摘　　要：利用SRAP标记,对来源不同的56份有棱丝瓜和8份无棱丝瓜种质的亲缘关系进行了分析。从144对SRAP引物中筛选出60对多态性强、重复性好的SRAP引物。共扩增得到1433条谱带,其中多态性谱带1280条,平均每对引物扩增得到21.33条多态性谱带,多态性位点百分率为88.74%。将丝瓜种质利用UPMGA方法进行聚类分析,结果表明,64份丝瓜种质的遗传相似系数为0.17～0.98。在遗传相似系数0.17处,供试种质被分为2大类群,第一大类群为普通丝瓜,第二大类群为有棱丝瓜,在第二大类群中又被分为以长绿型丝瓜为主和短果型丝瓜2亚群。丝瓜类群的划分与形态学性状比较一致,即首先与棱沟的有无密切相关,其次与瓜条的长短、颜色有较高的相关性。SRAP was used to analyse the genetic relationships of 56 angular sponge gourds and 8 sponge gourds from different areas. 60 pairs of polymorphic and stable primers were screened from 144 primer pairs. A total of 1433 DNA bands were amplified, 1280 of which were polymorphic. The average number of polymorphic DNA bands amplified by each primer pair was 21.33 and the ratio of polymorphic bands was 88.74%. UPMGA method was used to make cluster analysis and the result showed that the genetic similarity coefficient among 64 towel gourds germplasm ranged from 0. 17-0.98. The tested species were classified into 2 cluster groups with the similarity coeffi- cient of 0. 17. The first group was sponge gourd and the second group was angular sponge gourd with two sub-groups including long green types and short cyan types. Classification and morphological traits of towel gourds were relatively consistent, namely, they were closely related to have ridges or not at first, followed by fruit length and color.

关 键 词：丝瓜 种质资源 亲缘关系 SRAP 

分 类 号：S642.4[农业科学—蔬菜学]