基于ITS序列的不同产地裂叶荆芥系统发育分析  

Phylogenetic Analysis of Schizonepeta tenuifolia from Different Producing Areas Based on ITS Sequences

作　　者：刘红彬[1] 顾小龙[2] 张海红[1] 张丹参[1] 

机构地区：[1]河北北方学院药学系,张家口075000 [2]河北北方学院动物科技学院,张家口075000

出　　处：《植物遗传资源学报》2012年第6期1073-1077,共5页Journal of Plant Genetic Resources

基　　金：河北省中医药管理局课题(2011100);河北省教育厅课题(Z2010117)

摘　　要：采用PCR法扩增来自国内5个不同产地的裂叶荆芥ITS全序列,测序后以产自河北的裂叶荆芥代表中国裂叶荆芥与来自不同国家的裂叶荆芥ITS序列进行比较,构建分子系统发育树,探讨国内5个不同产地及不同国家的裂叶荆芥的亲缘关系和系统进化。结果表明:国内5个不同产地的裂叶荆芥ITS1和ITS2序列均有较高的G/C含量;5个产地的裂叶荆芥的扩增序列长度均为749bp,且序列完全相同;其中ITS1序列长231bp,5.8S序列长168bp,ITS2序列长236bp。中国裂叶荆芥与日本、韩国裂叶荆芥ITS序列一致性为100%,与美国裂叶荆芥ITS序列一致性为99.0%。与美国裂叶荆芥相比,中国裂叶荆芥ITS序列有7个碱基发生变异。来自不同国家的裂叶荆芥形成单系群和2个分支(中、日、韩3国为1个分支,美国单独形成1个分支)。ITS序列的一致性表明国内5个不同产地裂叶荆芥为同一个种。The genetic relationship and evolutionary system of Schizonepeta tenuifolia from 5 different produ- cing areas in China were analyzed based on the ribosome ITS1 and ITS2 sequences. Their complete ITS and 5.8S sequences on the ribosome region were amplified by PCR and were analyzed by software Clustal X （2.0） Multiple Sequence Alignments and Neighbor Joining. ITS sequence of S. tenuifolia from Hebei Province representing Chinese S. tenuifolia was compared with those from America, Japan, and Korea to construct phylogenetic tree. The results showed G/C contents of ITS1 and ITS2 sequences of S. tenuifolia from 5 different producing areas were high. ITS length was all 749bp for S. tenuifolia from 5 different producing areas with the same ITS sequence. Lengths of ITS1, 5.8S, and ITS2 were 231bp, 168bp, and 236bp respectively. The ITS sequence similarity was 100% among S. tenuifolia from China, Japan, and Korea, and 99% between those from China and America. Compared with S. tenuifolia from America, S. tenuifolia from China had 7 bases mutation. On the base of ITS sequence data, S. tenuifo- lia from different countries constructed a monophyletic group with 2 branches, one was consisted of S. tenuifolia from China, Japan, and Korea, the other one was only consisted of that from America. ITS sequence similarity suggested that S. tenuifolia from 5 different producing areas belonged to one species.

关 键 词：裂叶荆芥 产地 ITS PCR 测序 系统发育分析 

分 类 号：S567.219[农业科学—中草药栽培]