半胱天冬氨酸酶-3、相关X蛋白与溃疡性结肠炎辨证分型的相关性  

The expression and significance of Caspase-3/Bax in TCM differentiation of syndromes typing for ulcerative colitis

作　　者：王倩[1] 吕永慧[2] 周丽梅[2] 陈文剑[1] 江桂英[2] 江沂[2] 林调记[2] 

机构地区：[1]广东省广州市中医医院病理科,510130 [2]广东省广州市中医医院消化内科,510130

出　　处：《国际中医中药杂志》2012年第11期979-981,共3页International Journal of Traditional Chinese Medicine

基　　金：广州市中医药中西医结合科研立项课题（项目编号：2008802）

摘　　要：目的探讨溃疡性结肠炎（ulcerativecolitis，UC）中半胱天冬氨酸酶．3（Caspase．3）、B细胞淋巴瘤／白血病基因2相关X蛋白（Bax）的表达水平与辨证分型的相关性。方法将UC患者辨证分为湿热内蕴型组（25例）、脾胃虚弱型组（25例），用免疫组织化学技术（SP法），检测两组患者结肠黏膜腺上皮细胞Caspase．3、Bax的表达水平情况；并与正常对照组（25例）进行比较。结果Bax在湿热内蕴型[（4．56土1．58）分、脾胃虚弱型（3．28±1．14）分]的表达均升高，与正常对照组比较，差异均有统计学意义（p〈0．0．5）：两型的Caspase．3表达均升高，两组比较差异无统计学意义（P〉0．05）。此外，湿热内蕴型、脾胃虚弱型的Caspase．3、Bax的表达均呈正相关（r=0．23、r=0．21）。结论Bax、Caspase．3在UC中呈高表达，两者在细胞凋亡中具有调节作用，并与辨证分型有关。Objective To study the correlation between TCM differentiation of syndromes typing for ulcerative colitis （UC） and expressions of Bax and Caspase-3. Methods 50 patients with UC were divided into 2 groups according to TCM syndrome differentiation including dampness-heat internal accumulation syndrome group （25cases）, deficiency of spleen and stomach syndrome group （25cases）. Immunohistochemistry was used to study expression of Bax and Caspase-3 in colonic mucosas and compare with the normal group （25cases）. Results The expressions of Bax were highest in dampness-heat internal accumulation syndrome group （4.56±1.58）. The expressions of Bax of deficiency of spleen and stomach syndrome group were lower than dampness-heat internal accumulation syndrome group （3.28±1.14）scores. There were significant differences between the three groups （P〈0.05）. However, There were no significant difference between dampness-heat internal accumulation syndrome group and deficiency of spleen and stomach syndrome group in terms of the Caspase-3expressions （P〉0.05）. In addition, Caspase-3 and Bax were positively related in each group （r-0.23 and 0.21）. Conclusion The high expressions of Bax, Caspase-3 in ulcerative colitis were closely related to TCM typing according to syndrome differentiation. Caspase-3 and Bax were adjustment in apoptosis. The detection of expression of Bax, Caspase-3 is helpful to the TCM syndrome differentiation and to the selection of treatment plan. At the same time, it can provide more important experiment basses for the pathogenesis of ulcerative colitis.

关 键 词：半胱天冬氨酸酶 3 B细胞淋巴瘤 白血病基因2相关x蛋白 溃疡性结肠炎 辨证分型 

分 类 号：R259[医药卫生—中西医结合]