辛伐他汀对大鼠骨质疏松性骨折愈合的影响  

Effect of Simvastatin on Fracture Healing in Osteoporotic Rats

作　　者：田发明[1] 张柳[1] 骆阳[1] 宋亚琪[1] 杨方[1] 

机构地区：[1]河北联合大学,河北唐山063000

出　　处：《中国药学杂志》2012年第21期1719-1723,共5页Chinese Pharmaceutical Journal

基　　金：河北省自然科学基金资助项目(C2006000580)

摘　　要：目的观察骨质疏松对大鼠骨折愈合的影响及辛伐他汀对骨质疏松性骨折愈合的作用。方法 12周龄雌性Sprague-Dawley大鼠40只随机分成5组,每组8只:假手术组(A);卵巢切除组(B);正常骨折组(C);骨质疏松性骨折组(D);骨质疏松性骨折+辛伐他汀组(E)。除A、C组外,其余各组大鼠行双侧卵巢切除术,C、D、E组于卵巢切除术4周后制作右股骨中段骨折模型;E组给予辛伐他汀灌胃干预(20 mg.kg-1.d-1),C、D组给等量生理盐水。A、B组于术后4周处死,测量右股骨骨密度;其余3组于骨折后6周处死,完整取出右侧股骨,行CR摄片并评分、骨密度测定、HE染色并镜下组织学观察。结果①卵巢切除后4周,B组骨密度(BMD)显著低于A组(P<0.05);②各骨折组右股骨骨密度:D、E组tBMD、mBMD和dBMD均显著低于C组(P<0.05),E组各段骨密度均高于D组,但差异无统计学意义(P>0.05);③CR摄片:D组与E组整体愈合情况较C组差,多数标本骨折线清晰,X线评分均显著低于C组,E组高于D组,但差别无统计学意义;④组织学观察:C组大鼠骨痂组织更为成熟,可见板层骨形成,D组、E组软骨成分比例明显较高,均未见板层骨形成。结论骨质疏松大鼠骨折愈合较正常延迟,辛伐他汀可部分阻止去卵巢大鼠骨量丢失并表现出一定的促进骨折愈合的作用趋势,但效果并不显著。ABSTRACT ： OBJECTIVE To verify the delayed process of fracture healing in osteoporosis rats, as well as to investigate the effect of simvastatin on osteoprotic fracture healing. METHODS Fouty 12-week old female Sprague-Dawley rats were randomly divided into 5 groups with 8 animals in each group. All rats except those in group A and C rats received bilateral ovariectomy. The rats in group A received sham operation. The rats in group C, D and E underwent an operation 4 weeks after ovariectomy to establish the midshaft fe- mur fracture model all fractured rats were treated with simvastatin（group E, 20 mg~ kg ＇ ~ d ~） or vehicle（ group C,D） as control. Femurs from the A and B group rats sacrificed 4 weeks after ovaricctomy operation were harvested for the bone mineral density assess- ment. The fractured rats were sacrificed 6 weeks after fracture. Radiographic evaluation （ CR film） were taken to observe the fracture healing, a scoring system for CR fihn and bone mineral density were used to evaluate fracture healing quantitatively, and the femurs were then undecalcified for HE staining and subsequent histological observation. RESULTS Four weeks after the ovariectomy opera- tion, the BMD of rats in group B were significantly lower than those of group A（P 〈 0. 05 ） ; In contrast to group C, a significant de- creased BMD were observed in group D and E（P 〈0. 05） ; the rats in group E and D showed more clear fracture gap by CR films in most examples, indicating a delayed fracture healing process compared to group C, in which the rats showed more progressed callus consolidation, accordingly, the scores for evaluation of fracture healing in group C was significantly higher than those of group E and D （P 〈 0. 05 ） ; the rats in group C showed more mature callus with partially lamellar bone formation, while a delayed fracture healing process was observed in group D and E characterized by more cartilage callus but no lamellar bone formation group. CONCLUSION Compared to normal rats, osteoporot

关 键 词：辛伐他汀 骨密度 骨质疏松 骨折愈合 

分 类 号：R965[医药卫生—药理学]