掐茎尖和秋水仙素处理对核桃幼苗生长及倍性的影响  

Effects of top-pinching and colchicine on the seedlings growth and ploidy of walnut

作　　者：刘璐[1] 顾玉红[1,2] 李保国[3,2] 刘喜星[3] 刘洁[1] 张雪梅[3,2] 郭素萍[3,2] 班加骏[1] 

机构地区：[1]河北农业大学生命科学学院,河北保定071001 [2]河北省核桃工程技术研究中心,河北邢台054000 [3]河北农业大学林学院,河北保定071001

出　　处：《河北农业大学学报》2012年第5期29-33,共5页Journal of Hebei Agricultural University

基　　金：河北省科技支撑项目(11230115D)

摘　　要：以‘绿岭’核桃实生幼苗为材料,研究掐茎尖和秋水仙素对幼苗生长及倍性的影响。结果表明:处理C即未掐茎尖的核桃幼苗在第1～7天的高生长量为13.63cm,极显著高于处理C1(掐茎尖加蒸馏水处理)、C2～C6(掐茎尖加0.1%、0.2%、0.4%、0.6%、0.8%秋水仙素处理);各处理植株上胚轴上平均膨大的芽数以C1最高,为8.1个,极显著高于其他处理,表明掐茎尖和秋水仙素处理明显抑制核桃幼苗主茎生长,促进上胚轴上芽的萌发伸长;随着秋水仙素浓度的增加,植株上胚轴停长时间由处理第8～9天提前到第1天,植株顶端变黑萎蔫长度由切口发黑增大到2.48cm,上胚轴上平均萌发伸长的芽数由1.7个增加到3.0个;处理C2和C3出现二倍体细胞和四倍体细胞构成的混倍体叶片。＇Lv Ling＇ walnut seedlings were used as the experimental materials to study the effects of top-pinching and colehicine on the growth and ploidy of walnut seedlings. The results showed that： the height growth of ＇Lv Ling＇ walnut seedlings was 13.63 cm on the first to sev enth day by treatment C（no top-pinching）, significantly higher than that of Cl（top-pinching and dipped distilled water ）, C2-C6（top-pinching and treated with 0. 1%, 0. 2%, 0. 4%, 0. 6%, 0. 8% colchicine） . The average quantity of germinate buds was 8.1 in treatment C1, which was sig nificantly higher than that of others. It indicated that treating with top-pinching and colchicine inhibited growing of walnut seedlings, but it promoted the buds germination. With the increas ing of colchicine concentration, the time of seedlings stoped growing preset on the first dayfrom the eighth day to nineth day, dead tip extended from zero point to 2.48 cm on the epicot yl, the average quantity of buds on epicoty increased from 1.7 to 3. O. MixopIo^d leaf which constitute with diploid and tetraploid were found in C2 and C3.

关 键 词：核桃 掐茎尖 秋水仙素 生长 倍性 

分 类 号：S664.1[农业科学—果树学]