拟南芥AtCCaP2基因缺失突变体的鉴定及表型观察  

Identification and phenotype of T-DNA insert AtCCaP2 gene mutant in Arabidopsis thaliana

作　　者：高亢[1] 马惠斌[1] 吴立柱 潘延云[1] 

机构地区：[1]河北农业大学生命科学学院,河北保定071001

出　　处：《河北农业大学学报》2012年第5期67-71,共5页Journal of Hebei Agricultural University

基　　金：国家自然科学基金项目(No.30570993);河北省自然基金项目(C2008000292)

摘　　要：本研究拟利用反向遗传学研究AtCCaP2基因的功能。通过PCR及RT-PCR的方法筛选和鉴定了拟南芥AtCCaP2基因的T-DNA插入突变纯合体atccap2,该纯合体AtCCaP2基因表达缺失。RT-PCR分析显示该基因在拟南芥雌雄蕊等生殖器官中有较多的表达。通过对生长表型的观察发现atccap2突变体出现早花现象,抽薹时间较野生型早3d,显示该基因可能参与了拟南芥的早花表型。In this study, the function of AtCCaP2 gene was characterized using reverse genetics method. The T-DNA insert AtCCaP2 gene mutants were identified by PCR and RT-PCR, and the homozygote mutant atccap2 was isolated. RT-PCR analyses showed that AtCCaP2 gene were higher expression in stamen and pistil than in leaf. The atccap2 mutants were early bol ting 3 days than the wild type. These result showed that AtCCaP2 gene might cause early flowering phenotype in Arabidopsis.

关 键 词：拟南芥 AtCCaP 反向遗传学 

分 类 号：Q789[生物学—分子生物学]