高海拔地区牦牛心肌骨骼肌ATP酶活性的测定  

High altitude yak cardiac and skeletal muscle mitochondria ATP activity is determined

作　　者：王勇[1] 李莉[1] 沈明华[1] 荆海霞[1] 张勤文[1] 

机构地区：[1]青海大学农牧学院,青海西宁810016

出　　处：《中国兽医杂志》2012年第10期28-30,共3页Chinese Journal of Veterinary Medicine

基　　金：青海省科技厅资助项目(2010-Z-745);国家自然科学基金项目(31160489)

摘　　要：为了探讨高原动物对高原低氧的适应机制,采用标准ATP酶测试盒对青海省(平均海拔4 000m)16头成年牦牛心肌、骨骼肌ATP酶活性进行了测定和分析。结果:Na+-K+-ATP,Mg2+-ATP,Ca2+ATP和Ca2+-Mg2+-ATP 4种酶活性在骨骼肌线粒体中分别为3.65±1.31(μmolP/mg蛋白.h,下同),2.70±1.36,2.87±1.23和4.08±1.30;在心肌线粒体中分别为4.44±1.19,2.42±0.87,2.29±0.57和3.62±0.92;在骨骼肌组织中分别为2.02±0.80,1.54±0.36,1.69±0.91和2.02±0.96;在心肌组织中分别为1.41±0.67,1.04±0.51,1.24±0.54和1.42±0.60;骨骼肌、心肌线粒体中4种ATP酶活性均显著高于骨骼肌、心肌组织(P<0.05)。In order to research the plateau on the plateau animals adapt to the low oxygen mechanism at high altitude, The activity of ATP in cardiac and skeletal muscle mitochondria in Zeku and Maduo coun- ty of Qinghai province 16 adult yak were determined by ATP determination Kit. The result of Na＋-K＋- ATP, Mg2＋-ATP, Ca2＋-ATP and Ca2＋-Mg2＋-ATP activity in skeletal muscle mitochondria were 3.65_＋ 1.31 （μmolP/mgprot/h）. the same below）,2.70!1.36,2.87±1.23 and 4.08±1.30;The result of Na＋- K＋ -ATP, Mgz＋-ATP, Ca2＋-ATP and Caz＋ -Mg2＋-ATP activity in cardiac and skeletal muscle mitochondria were 4.44＋1. 19,2.42±0.87,2.29±0.57 and 3.62±0.92. The result of Na＋-K＋-ATP, Mg2＋-ATP, Ca2＋-ATP and Ca2＋-Mg2＋-ATP activity in skeletal muscle tissue were 2.02 ± 0.80,1.54 ! 0.36,1.69 ± 0.91 and 2.02 ± 0.96 ;The result of Na＋ -K＋-ATP, Mg2＋ -ATP, Ca2＋ -ATP and Ca2＋-Mg2＋ -ATP activity in cardiac and skeletal muscle tissue were 1.41!0.67,1.04±0.51,1.24!0.54 and 1.42±0.60. The result showed the activity of ATP in cardiac and skeletal muscle mitochondria were higher significantly than thatof tissue （P〈0. 05）.

关 键 词：牦牛 心肌 骨骼肌 线粒体 ATP酶 

分 类 号：S823.85[农业科学—畜牧学]