黄芩素抑制流感病毒A/FM1/1/47(H1N1)的一个新机理:干扰病毒中后期mRNA的合成(英文)  

A novel inhibitory mechanism of baicalein on influenza A/FM1/1/47 (H1N1) virus: interference with midlate mRNA synthesis in cell culture

作　　者：苏真真[1] 窦洁[1] 徐志鹏[1] 郭青龙[2] 周长林[1] 

机构地区：[1]中国药科大学生命科学与技术学院,南京210009 [2]中国药科大学药学院,南京210009

出　　处：《中国天然药物》2012年第6期415-420,共6页

基　　金：supported by the grants from the National Science and Technology Major Project Foundation of China (No. 2011ZX09102-009-05);the Fundamental Research Funds for the Central Universities (No. JKP2011018);the "111 Project"from the Ministry of Education of China and the State Administration of Foreign Expert Affairs of China (No. 111-2-07)~~

摘　　要：目的:阐明黄芩素在细胞培养中对流感病毒A/FM1/1/47(H1N1)的抑制机理。方法:通过结晶紫染色实验测定黄芩素对流感病毒的抑制作用,采用不同加药方式进一步研究黄芩素对病毒复制周期不同阶段的影响。通过RT-PCR方法比较黄芩素给药组和病毒模型组流感病毒M1基因mRNA的水平,确定黄芩素对病毒mRNA合成的抑制作用。结果:不同浓度的黄芩素在体外能够明显抑制流感病毒A/FM1/1/47(H1N1)和A/Beijing/32/92(H3N2)的感染,并呈剂量依赖。病毒感染后再加入药物黄芩素(治疗法)的抑制作用明显好于病毒感染前(预防法)和病毒感染同时(同时法)加入的抑制效应。在病毒感染6h、9h时,黄芩素给药组的病毒M1基因mRNA水平明显降低。结论:黄芩素体外可通过干扰流感病毒A/FM1/1/47(H1N1)中后期mRNA的合成抑制病毒的复制。AIM： To elucidate the inhibitory mechanism of baicalein on influenza A/FM1/1/47 （H1N1） virus in cell culture. METHODS： The inhibition of baicalein on influenza virus infection was investigated using a crystal violet staining assay. Three infection protocols were used to study the inhibitory effects of baicalein on different stages of the replication cycle of the influenza virus. Viral M1 mRNA expression was compared between drug-treated and untreated infected cells to determine the inhibitory effect of baicalein on viral mRNA synthesis using RT-PCR. RESULTS： Both the A/FM1/1/47 （H1N1） and A/Beijing/32/92 （H3N2） influenza viruses were inhibited with different concentrations of baicalein in a dose-dependent manner. Treatment with baicalein after viral inoculation to MDCK cells resulted in stronger inhibitory effects compared with treatments prior to, and at the same time, as viral inoculation. Furthermore, no significant difference was observed between the viral M1 mRNA synthesis in the baicalein-treated group and the untreated group at 3 hpi （hours post-infection）, compared to a clear decrease of viral mRNA synthesis at 6 and 9 hpi. CONCLUSION： This study has identified a novel inhibitory mechanism of baicalein on influenza A/FM1/1/47 （H1N1） virus via interference with mid-late mRNA synthesis in cell culture.

关 键 词：黄芩素 流感病毒 抗病毒机理 mRNA合成 

分 类 号：R965[医药卫生—药理学]