高氧对新生大鼠肠道分泌片表达的影响  

Changes of intestinal secretory component in neonatal rats by heperoxia

作　　者：刘冬妍[1] 姜腾[1] 王思[1] 曹旭[1] 

机构地区：[1]中国医科大学附属盛京医院实验研究中心(卫生部小儿先天畸型重点实验室),辽宁沈阳110004

出　　处：《中国现代医学杂志》2012年第26期5-8,共4页China Journal of Modern Medicine

基　　金：国家自然科学基金(No:30871158;81170604);盛京自由研究者基金

摘　　要：目的研究高氧对新生大鼠肠道分泌片表达的影响。方法采用免疫组织化学和Weastern blotting检测高氧对新生大鼠肠黏膜分泌分泌片的影响。结果 SC染色可见正常肠组织肠上皮细胞浆内和膜上呈黄色阳性,高氧组染色明显加强。Weastern blotting结果显示高氧组SC OD值/β-actin OD值第14天是(0.919±0.060)、第21天是(0.934±0.044)。空气对照组SC OD值/β-actin OD值第14天是(0.702±0.032)、第21天是(0.704±0.022)。高氧组新生大鼠肠组织表达分泌片较空气对照组明显升高(P<0.01)。结论在持续高氧作用下新生大鼠肠黏膜分泌大量分泌片,这可能有利于维护肠道免疫屏障的完整。【Objective】 To study the changes of intestinal secretory component（SC） in neonatal rats by heperoxia.【Methods】 Intestinal secretory component in neonatal rat by inhaling hyperoxia and air were assayed by immunohistochemistry and Western blotting.【Results】 The result of immunohistochemistry showed that the expression of SC in the cytoplasm and on the membranes of intestinal epithelial cells in hyperoxia group was remarkably enhanced than in the air-inhaling group.The result of Western blot showed that in hyperoxia group OD value of SC/OD value of β-actin was（0.919±0.060） at 14th day and（0.934±0.044） at 21st day.In air-inhaling group OD value of SC/OD value of β-actin was（0.702±0.032） at 14th day and（0.704±0.022） at 21st day.Relation rate of hyperoxia group was notably increased compared with relation rate of air-inhaling group in the 14th day and 21st day（P 0.01）.【Conclusion】 A lot of intestinal secretory component was secreted in neonatal rats by heperoxia to maintain intestinal immunity barrier.

关 键 词：高氧 肠道 分泌片 

分 类 号：R333.3[医药卫生—人体生理学] R-332[医药卫生—基础医学]