高效液相色谱法测定草甘膦母液中甲醛含量  

Determination of Formaldehyde in Glyphosate Mother Liquor by HPLC

作　　者：孙鸿曼[1] 任树杰[1] 尹荃[1] 闫海生[1] 

机构地区：[1]沈阳化工研究院有限公司生物技术研究室,沈阳110021

出　　处：《农药》2012年第11期809-810,共2页Agrochemicals

摘　　要：[目的]应用衍生化-高效液相色谱法建立草甘膦母液中甲醛含量的分析方法。[方法]样品中甲醛与2,4-二硝基苯肼衍生反应,生成的2,4-二硝基苯腙用高效液相色谱进行快速分离,在反相C8色谱柱、流动相为乙腈-水（体积比55∶45）、流速1.0 mL/min、紫外检测波长360 nm条件下进行外标定量。[结果]该分析方法在30~300 mg/L质量浓度范围呈良好线性,添加回收率在86.72%~94.12%之间,相对标准偏差小于5%。[结论]该方法应用于草甘膦母液中甲醛的测定,操作简便,结果准确。[Aims] A method for the determination of formaldehyde in glyphosate mother liquor by offline derivatization-high performance liquid chromatography（HPLC） was established.[Methods] The sample was derivatized with 2,4-dinitrophenyl hydrazine.The derivatized product was separated on a Agilent C8 column at 360 nm with mobile phase consisting of acetonitrile-water（55∶45,by vol） at the flow rate of 1.0 mL/min and quantified by external standard method.[Results] The method was validated from 30 to 300 mg/L with satisfied linear relationship.The recovery rates were between 86.72 to 94.12%,with less than 5% of relative standard deviation.[Conclusions] The method was shown to be simple,rapid and practical.

关 键 词：甲醛 草甘膦 甲醛衍生物 2 4-二硝基苯肼 高效液相色谱 

分 类 号：TQ450.7[化学工程—农药化工]