裸仁美洲南瓜白粉病菌对几种杀菌剂敏感性及抗药性诱导  

Fungicides sensitivity of Podosphaera xanthii and its resistance induction on hull-less pumpkin

作　　者：梁巧兰[1] 魏列新[1] 徐秉良[1] 

机构地区：[1]甘肃农业大学草业学院,草业生态系统教育部重点实验室,中-美草地畜牧业可持续发展研究中心,甘肃省草业工程实验室,兰州730070

出　　处：《农药》2012年第11期840-843,849,共5页Agrochemicals

基　　金：国家自然基金项目(30671267,31060261);甘肃省科技厅自然科学基金项目(1010RJZA164)

摘　　要：[目的]研究南瓜白粉病菌对几种杀菌剂敏感性及抗药性产生情况。[方法]防效试验采用室内盆栽方法和离体叶片含药培养基法。[结果]筛选出10%苯醚甲环唑WG、62.5%锰锌.腈菌唑WP、5%腈菌唑EC、2%农抗120 AS、70%甲基托布津WP和5%己唑醇ME 6种杀菌剂对南瓜白粉病菌有较好防效。接种后不同施药时间能够影响南瓜白粉病菌对杀菌剂的敏感性。[结论]筛选出的6种杀菌剂防效均在80%以上;几种药剂在不同时间对南瓜白粉病的EC50值测定结果表明:5%腈菌唑EC和70%甲基托布津WP、5%己唑醇ME和62.25%锰锌.腈菌唑WP、2%农抗120 AS分别在接菌后48、96、120 h施药较为敏感,而10%苯醚甲环唑WG和70%甲基托布津WP在接菌前24 h施药敏感;连续2次使用相同杀菌剂防治南瓜白粉病菌后,病原菌对5%己唑醇ME产生了轻度抗性,抗性倍数为6.79。[Aims] The objective of this paper is to study the sensitivity of Podosphaera xanthii on hull-less pumpkin and its resistance generation to some fungicides.[Methods] Control effects of several fungicides to P.xanthii were tested by indoor pot control effects test method and detached leaves-brushing with conidial suspension and medium with fungicides method.[Results] Six kinds of fungicides difenoconazole 10% WG,mancozeb＋myclobutanil 62.5% WP,myclobutanil 5% EC,TF-120 2% AS,thiophanate-methyl 70% WP and hexaconazole 5% me had good control effects on pumpkin powdery mildew and their sensitivity of pumpkin powdery mildew were affected in different application time.[Conclusions] The control effects of six kinds of fungicides all reached more than 80%.the EC50 values of difenoconazole,mancozeb＋myclobutanil,myclobutanil,TF-120,thiophanate-methyl and hexaconazole to P.xanthii treated in different time after inoculation were determined,the results showed that the sensitive time of myclobutanil and thiophanate-methyl,hexaconazole and mancozeb＋myclobutanil,TF-120 to P.xanthii on hull-less pumpkin were 48,96 and 120 h respectively after inoculation;while the sensitive time of difenoconazole and thiophanate-methyl were 24 h before inoculation.Using the same fungicide repeatedly could induce the light resistance of P.xanthii to hexaconazole,the resistance factor was 6.79.

关 键 词：裸仁美洲南瓜 白粉病菌 杀菌剂 敏感性 

分 类 号：S482.2[农业科学—农药学]