双酚A对萼花臂尾轮虫毒性及生活史的影响  

Effects of bisphenol A on the toxicity and life history of the rotifer Brachionus calyciflorus

作　　者：陆正和[1,2] 赵宝坤[1] 杨家新[1] 

机构地区：[1]南京师范大学生命科学学院,南京210046 [2]淮海工学院海洋学院,连云港222005

出　　处：《生态学报》2012年第21期6828-6835,共8页Acta Ecologica Sinica

基　　金：国家自然科学基金项目(30570260);江苏省科技支撑计划(农业)项目(BE2011366)

摘　　要：应用生态毒理学研究方法,研究了双酚A(BPA)对萼花臂尾轮虫毒性及生活史影响。结果表明:BPA对萼花臂尾轮虫24h急性毒性LC50为13.76 mg/L,95%置信限为10.97—17.10 mg/L。生活史研究显示:与对照组相比,0.25—4.0 mg/L的BPA对轮虫胚胎发育历时影响不显著;2.0—4.0 mg/L的BPA显著延迟了轮虫幼体发育时间;当BPA为0.5 mg/L时,轮虫净生殖率(R0)显著上升,但BPA浓度为2.0和4.0 mg/L时R0显著降低;4.0 mg/L的BPA显著缩短世代时间(T);BPA浓度为2.0、4.0 mg/L时,轮虫生命期望值(e0)显著下降;BPA浓度为1.0、2.0、4.0 mg/L时,轮虫内禀增长率(rm)显著下降;BPA浓度为0.5、1.0 mg/L时轮虫所产后代混交率(MR)显著增长。研究结果表明BPA对轮虫的生殖具有干扰作用,其中R0和MR受BPA影响最为显著。The presence of toxic agents in aquatic environments has increased in recent years. Some of them that are released into the aquatic environment interfere with the endocrine system of organisms by affecting development time, causing reproductive abnormalities and reducing population growth rates in zooplankton including rotifers. Bisphenol A （BPA, 4,4Z-isopropylidine diphenol ） is a commercially important chemical with an estimated worldwide production capacity of 3.7 million metric tons per year and demonstrates estrogenic activity. Many acute and chronic toxicity studies under controlled conditions have used either fish or the cladoceran Daphnia magna. However there is little information using rotifers with BPA. With short life spans and high reproductive abilities, rotifers such as Brachionus calyciflorus are particularly useful for environmental toxicology because of their higher sensitivities to most toxicants. Moreover, rotifers are important trophic components of aquatic food webs constituting a major food source for fish and invertebrate predators and thus play a vital role in transporting energy and nutrients in aquatic ecosystems. The aim of the present work was therefore to use B. calyciflorus to study the effect BPA on the life history characteristics and mixis. Population of B. calyciflorus was （0--2 h old） obtained by hatching resting eggs in EPA medium. Resting eggs were endowed by professor T W. Snell. Test toxicant solutions were prepared by US EPA medium. The rotifers were fed using unicellular green algae Chlorella pyrenoidosa at 1.0x 106 cells/mL, the alga was cultured in a semi-continuous culture using HB-4 medium in 3 L transparent flasks under a light intensity of 4000 lux and photo period was 12：12 （L：D）. The results showed that 24 h median lethal concentration （24 h LC50 ） value of BPA for B. calyciflorus was 13.76 mg/L, and 95% limits was 10.97--17. 10 mg/L. In the tested eoncentration range of 0.25--4. 0 mg/L, BPA at 2.0 and 4.0 mg/L extended signifieantly the durati

关 键 词：萼花臂尾轮虫 双酚A 环境激素 生活史参数 混交率 

分 类 号：S963.214[农业科学—水产养殖]