职业接触苯乙烯工人静脉血中蛋白质环氧苯乙烯加合物的分析测定  

Detection of protien styrene oxide adducts in human blood exposed to styrene

作　　者：刘淑芬[1] 方清明[1] 金祖亮[1] Rappaport S.M 

机构地区：[1]中国科学院生态环境研究中心,北京100085 [2]UNCATCHAPELHILLNCUSA

出　　处：《环境科学学报》2000年第4期461-466,共6页Acta Scientiae Circumstantiae

基　　金：国家自然科学基金!(批准号 :5 98730 2 8);美国卫生基金

摘　　要：静脉血中蛋白质 环氧苯乙烯 (SO)加合物的分析测定是将蛋白质中半胱氨酸残留经分解后 ,用RaneyNi催化反应生成两种加合物 :α 苯己醇 ,β 苯已醇 ,再用五氟苯酰氯衍生 ,GC/MS测定 .用同样的方法测SD大鼠血的蛋白质加合物 ,结果表明动物血中蛋白质加合物与实验的苯乙烯或SO剂量有较好的相关性 ;而且SO与半胱氨酸的反应α位强于 β位 .在人群实验中 ,对一个石棉厂职业接触苯乙烯工人静脉血中的蛋白质加合物进行测定 ,结果血红蛋白与SO的α位、α位 +β位的加合物与个体接触苯乙烯浓度有一定的相关 ,β位的加合物不明显 ;白蛋白中的加合物与接触苯乙烯也有一定的相关 .为探索用人血蛋白质中的半胱氨酸 SO加合物作为人接触苯乙烯的生物标志物提供依据 .An assay is described for measures of adducts of styrence oxide (SO),with molecules of protein which have been modified at residues containing sulhydryl group.Sample is hydrolyzed,then and reacted with Raney Ni to liberate the two positionisomer of SO\|cysteine adducts,i.e.,1\|phenylethanoland,2\|phenylethanol.The liberated products are derivatized with pentafluorobenzoyl chloride and measured by GC/MS.The assay was evaluated with SO adducts of Hb which had been produced in blood of SD rat and in hurman blood exposed to styrene.Level of protein\|SO adducts in rat blood increased with dose and the α\|position rather that the β\|position both.The two adducts were found in workers blood exposed to styreneand there is a good relativity between adduct level and exposed dose of styrene..It is suggested that SO\|protein adduct can be a biomarker of styrene exposure in workers.

关 键 词：蛋白 加合物 苯乙烯 环氧苯乙烯 人体污染监测 

分 类 号：X838[环境科学与工程—环境工程] R135.12[医药卫生—劳动卫生]