表达CSFV E2蛋白重组AcNPV载体的构建  

Construction of Recombinant AcNPV Expressing CSFV E2 Protein

作　　者：胡刚叶[1] 苌生科[1] 庄金山 白朝勇 张许科[1,2] 

机构地区：[1]河南农业大学动物传染病与微生物实验室,郑州450002 [2]国家兽用药品工程技术研究中心,河南洛阳471000

出　　处：《中国兽药杂志》2012年第11期1-5,共5页Chinese Journal of Veterinary Drug

摘　　要：利用基因重组技术成功构建两株含猪瘟兔化弱毒株E2基因的重组苜蓿银纹夜蛾核型多角体病毒(AcNPV),重组病毒能够在昆虫细胞中分泌表达CSFV E2蛋白。将重组病毒注射入家蚕蛹内,于注射后第7天收获蚕蛹血淋巴并进行Western-blot鉴定目的蛋白的表达。结果显示,重组病毒在家蚕蛹中成功表达了CSFV E2蛋白。本研究为进一步开发猪瘟亚单位疫苗和诊断试剂盒用抗原提供了新的途径。In this study, we constructed two recombinant AcNPVs, containing CSFV E2 gene, which could secrete CSFV E2 protein in infected Sf9 cells. Then, the recombinant viruses were injected into silkworm chrysalis and hemolymph was collected 7 days after injection for western - blot analysis of CSFV E2 protein. The results showed that CSFV E2 protein was expressed successfully in silkworm chrysalis. This work was expected to provide a new way for expressing CSFV E2 protein.

关 键 词：猪瘟病毒 E2蛋白 ACNPV 蚕蛹 

分 类 号：Q786[生物学—分子生物学]