功能菌群耦合黄铁矿浸出软锰矿的研究  

Study of coupled pyrite leached pyrolusite by functional microflora

作　　者：马皛梅 韩一凡[2] 路福平[1] 张小霞[2] 温丹[2] 刘勇金 白利明 胡影 黄志勇[2] 

机构地区：[1]天津市工业微生物重点实验室、工业发酵微生物教育部重点实验室、天津科技大学生物工程学院,天津300457 [2]天津市工业生物系统与过程工程重点实验室、中国科学院天津工业生物技术研究所,天津300308 [3]河北恒基锰业有限公司,河北唐山064300

出　　处：《微生物学通报》2012年第11期1551-1559,共9页Microbiology China

基　　金：国家863计划项目(No.2012AA021203);财政部战略生物资料源科技支撑运行专项项目(No.KSCX2-YW-Z-1016);唐山市科学技术研究与发展计划课题基金项目(No.08110207A-2)

摘　　要：【目的】将3种不同来源的环境样品混合后接种至含1%黄铁矿和1%软锰矿的培养基中进行富集培养,初步得到有一定浸矿功能的混合微生物菌群。【方法】菌群继续用于黄铁矿和低品位软锰矿共同浸出,设置未接种的体系作为对照。【结果】对浸出过程中菌群结构的变化、pH、锰浸出率和浸出残渣的成分进行分析,结果发现接种过微生物菌群的浸出体系在反应15 d后,锰浸出率达到92.48%,远高于未接菌对照组的40.34%;菌群中Thiomonas sp.所占比例从最初的2%上升到浸出结束时的93%。实验组的pH从最初的4.0下降到2.5;X射线衍射(XRD)分析发现,通过生物作用浸出的残渣中含有黄钾铁矾,说明生物代谢产生了大量的硫酸。【结论】证明微生物在两矿浸出过程中通过促进黄铁矿解离,维持体系低pH等作用加速反应的进行。结果为进一步研究微生物浸矿的作用机制和开发低品位锰矿的生物浸出工艺打下了基础。[Objective] Three different environmental samples were mixed and inoculated into 100 mL fresh medium containing 1% pyrolusite and 1% pyrite. After enrichment we got bioleaching microflora. [Methods] Obtained microorganism consortia were used to leach py- rite and low-grade pyrolusite. Non-inoculation system was conducted as control group. [Results] Series of parameter were monitored during the process, including dynamic of consortia construction, pH value, leaching ratio of manganese, and XRD analyze of leaching residual. Leaching ratio of manganese was 92.48% after 15 days cultivation. And it was much higher than control group （40.34%）. Within microbial consortia, Thiomonas sp. was the dominant species and its proportion increased from 2% to 93% of the consortia. Value of pH was de- clined from 4.0 to 2.5 in experimental group. Jarosite was observed in bioleaching residual. [Conclusion] Decrease of pH value and formation ofjarosite indicated abundant sulfuric acid produced by bacterial metabolism. Result suggested that increasing bioleaching ratio of pyrite and pyrolusite due to dissociation of pyrite and decline of pH value by bacterial activity. This study could provide the basis for the further research of bioleaching mechanism and improve- ment of industrial art of low-manganese ore mineral resource.

关 键 词：混合功能菌群 黄铁矿 软锰矿 细菌浸出 16S rRNA基因文库 

分 类 号：TD951[矿业工程—选矿] TD925.5