巴尔通体液体培养条件简化及生长曲线观察  

Optimization of liquid growth conditions and determination of growth curves for Bartonella species

作　　者：栗冬梅[1] 苗志刚[2] 宋秀平[1] 王君[1] 刘起勇[1] 

机构地区：[1]中国疾病预防控制中心传染病预防控制所传染病预防控制国家重点实验室北京102206 [2]山东大学生命科学院山东济南250100

出　　处：《微生物学通报》2012年第11期1695-1702,共8页Microbiology China

基　　金：国家自然科学基金项目(No.81101286)

摘　　要：【目的】应用一种昆虫细胞培养基作为基础成分培养巴尔通体(Bartonella species),建立一种操作方便、高效稳定的巴尔通体液体培养方法。【方法】昆虫细胞培养基中添加10%胎牛血清,以此为基础培养液分别添加蔗糖和谷氨酰胺,比较这两种成分对汉赛巴尔通体(B.henselae)和五日热巴尔通体(B.quintana)生长的影响并观察其他10种巴尔通体在简化后的培养液中的生长特性。【结果】添加蔗糖和谷氨酰胺不会明显促进巴尔通体的生长,10种巴尔通体在简化后的培养液中均生长良好。不同种巴尔通体生长曲线不同,汉赛巴尔通体和五日热巴尔通体的世代时间分别为5.2 h和4.3 h,生长速度快于固体培养。【结论】以昆虫细胞培养基作为基础成分的培养液适于巴尔通体液体培养,特别是对一些更难培养的巴尔通体提供了一种较好的培养方法。[Objective] We tested an insect cells-based liquid medium and its utility for the easy-to-maintain, fast and reliable system for cultivation of different Bartonella. llvJetnoosl Media composition and growth conditions were optimized using B. henselae and B. quintana. The growth curves of the other ten Bartonella species were determined using the optimized liquid medium. [Results] Glutamine and sucrose supplements did not affect significantly the growth rate of these bacteria. A generation time of 5.2 h for B. henselae and 4.3 h for B. quintana was estimated during the exponential phase of the growth curve occurred between days 1 and 2 after inoculation. The twelve medical and veterinary important Bartonella species grew well under conditions established and each exhibited unique growth characteristics. [Conclusion] This liquid growth medium may provide an advantage over conventional direct blood agar plating for analyzing Bartonella properties including antibiotic susceptibility and resistance and pathogenicity.

关 键 词：巴尔通体 液体培养 生长曲线 猫抓病 

分 类 号：R378[医药卫生—病原生物学]