HPLC测定怀菊花中的总黄酮  

Determination of Total Flavonoids in Chrysanthemum by HPLC

作　　者：斯琴格日乐[1] 范云场[1] 恩德[1] 马纪[1] 肖华伟[1] 

机构地区：[1]河南理工大学理化学院

出　　处：《光谱实验室》2012年第6期3342-3345,共4页Chinese Journal of Spectroscopy Laboratory

基　　金：国家自然科学基金项目(41074090);河南省科技计划重点攻关项目(092102210360)

摘　　要：建立了HPLC测定怀菊花中总黄酮含量的方法。采用Agilent C18柱(150mm×4.6mm,5μm),以甲醇(A)-超纯水(0.2%的冰乙酸)(B)(25:75,V/V)为流动相,流速:0.5mL·min-1;柱温为20℃,检测波长:260nm;进样量:10μL。结果:回归方程为y=33.29x-8.284,r=0.9993(n=6),线性范围0—20μg,平均回收率为102.63%,RSD为5.56%。样品总黄酮含量为7.51mg/g。方法简便、灵敏、快速、准确、重现性好,可用于怀菊花中总黄酮含量的质量控制。The method for determination of content of total flaconoids in chrysanthemum by HPLC was established. The compound was separated by Agilent C18 column （150mm × 4.6mm, 5μm） with methanol（A）-ultra-pure water （0. 2% acetic acid）（B）（25 ：75,V/V） as mobile phase at a flow rate of 0. 5mL· min-1, and column temperature was 20℃,detection wavelength was 260nm and injection volume was 10μL. The regression equation was y=33.29x--8. 284,r=0. 9993（n=6）,and the linear range was from 0 to 20μg,the average recovery was 102. 63% with RSD of 5.56%. The content of total flavonoids in the sample was 7.51mg/g. The method is simple,sensitive, rapid and accurate with good reproducible,and can be applied to quality control of the content of total flavonoids in chrysanthemum.

关 键 词：高效液相色谱法 怀菊花 总黄酮 

分 类 号：O657.72[理学—分析化学]