锌原卟啉与血红素对弥漫性脑外伤大鼠耳蜗血红素加氧酶表达及听性脑干反应影响的实验研究  

The effects of Zinc Protoporphyrin and Heme on the expression of HO-1 in cochlear and the change of audi- tory brainstem response on diffuse traumatic brain injury model of rats

作　　者：付兴华[1] 张萍[1] 邓艳丽[1] 卢鹤翔[3] 孙瑞军[3] 刘颖[2] 赵君晖[1] 顾洪兰[1] 韩莉[1] 张再兴[1] 

机构地区：[1]河北联合大学附属医院耳鼻咽喉科,河北省唐山063000 [2]河北联合大学附属医院肿瘤科,河北省唐山063000 [3]河北联合大学形态中心

出　　处：《中国基层医药》2012年第22期3361-3364,I0001,共5页Chinese Journal of Primary Medicine and Pharmacy

基　　金：基金项目：河北省科学技术研究与发展计划项目（08276101D-74）

摘　　要：目的探讨锌原卟啉（Znpp）与血红素（Heme）对弥漫性脑外伤大鼠耳蜗血红素加氧酶（HO-1）表达及听性脑干反应（ABR）的影响。方法建立弥漫性脑外伤大鼠模型并随机分组，采用ABR测定、光镜、免疫组织化学等方法，观察各组动物耳蜗HO-1表达及ABR的变化。结果外伤后各组ABR阈值及各波潜伏期差异均有统计学意义（均P〈0．05）。对照组大鼠耳蜗HO-1无表达，外伤后各组耳蜗HO．1表达有明显变化（P〈0．01）。正常对照组与DBI组、Znpp、Heme各组灰度值差异均有统计学意义（均P〈0．05）。结论锌原卟啉与血红素对弥漫性脑外伤大鼠耳蜗HO-1表达及ABR均有不同程度的影响，血红素对听功能的保护作用可能与HO—1表达升高有关。Objective To explore the effects of Zinc Protoporphyrin and Heme on the expression of HO-1 in cochlear and the change of auditory brainstem response on diffuse traumatic brain injury model of rats. Methods Dif- fuse traumatic brain injury model of rats were established and randomly divided into thirteen groups. Then auditory brainstem response examination,light microscope, immunohistochemistry technique were used to evaluate the change of auditory brainstem response and the expression of HO-1 in cochlear. Results The differences of auditory brainstem response threshold and latency of wave between the experimental and the normal control group were obvious （P 〈 0. 05 ）. The expression of HO-1 in the control group was normal, whereas there were obvious changes of inner ear HO- 1 expression in the traumatic groups. The grey value of HO-1 expression in trauma group, Znpp group and heine group was significantly associated with auditory function change （ P 〈 0.05 ）. Conclusion There were influence of Zinc Pro- toporphyrin and Hemeon the inner ear HO-1 expression and the change of auditory brainstem response with diffuse traumatic brain injury model of rats. The protective effect of heme on auditory function may be associated with the in- creased expression of HO-1.

关 键 词：弥漫性脑外伤 锌原卟啉 血红素 耳蜗 血红素加氧酶 听性脑干反应 

分 类 号：R651.1[医药卫生—外科学]