青蒿素对骨关节炎大鼠肿瘤坏死因子-α表达的影响  

Effect of arteannuin on expression of tumor necrosis factor-α in rats with ostarthritis

作　　者：何文彤[1,2] 单娜娜[3] 董化江[1,2] 杨徳慧 李伯森 单云官[1,2] 

机构地区：[1]武警后勤学院人体解剖学教研室 [2]民政部101研究所遗体防腐整容重点实验室,天津300162 [3]武警后勤学院教育技术中心,天津300162 [4]民政部101研究所,北京100070

出　　处：《新乡医学院学报》2012年第11期813-814,817,共3页Journal of Xinxiang Medical University

基　　金：国家十一五科技支撑计划课题(编号:2007BAK38B05)

摘　　要：目的探讨青蒿素对骨关节炎大鼠肿瘤坏死因子-α(TNF-α)的影响。方法 32只骨关节炎模型大鼠随机分为对照组和青蒿素高剂量组、中剂量组、低剂量组,每组各8只,对照组给予生理盐水20 mL灌胃,每日1次,青蒿素高、中、低剂量组分别给予青蒿素400、300、200 mg.kg-1(均溶于20 mL生理盐水)灌胃,每日1次,连续7周,实验结束后右心房取血,放射免疫法检测血清TNF-α水平,反转录聚合酶链式反应、蛋白质印迹检测关节软骨组织中TNF-α的表达。结果青蒿素低、中、高剂量组血清TNF-α水平均较对照组下调(P<0.05),低剂量组水平高于中、高剂量组(P<0.05),中剂量组水平高于高剂量组(P<0.05);低剂量组组织mRNA水平与中剂量组比较差异无统计学意义(P>0.05),高剂量组的组织mRNA水平低于低、中剂量组和对照组(P<0.05);组织TNF-α蛋白水平中剂量组与高剂量组比较差异无统计学意义(P>0.05),但均低于对照组和低剂量组(P<0.05)。结论青蒿素可降低关节炎大鼠TNF-α的表达,有效控制炎症反应进程。Objective To observe the effect of arteannuin on the level of tumor necrosis factor-α（TNF-α） in rats with ostarthritis. Methods Thirty-two rats with ostarthritis were randomly divided into control group,high-dose arteannuin group, media-dose arteannuin group and low-dose arteannuin group, eight rats in each group. The rats in control group were given with saline 20 mL by garage, once a day. The rats in high-close arteannuin group, media-dose arteannuin group and low-dose artean- nuin group were given with arteannuin 400,300 and 200 mg·kg- 1 respectively by gavage, once a day for seven weeks （the ar- teannuin was dissolved in 20 mL saline）. The level of TNF-α was detected by radioimmunoassay（RIA） ,and the expressions of TNF-α mRNA and protein were detected by reverse transcription-polymerase chain reaction and Western blotting. Results The levels of serum TNF-α in high-dose, media-dose and low-dose arteannuin groups were decreased significantly than that of control group（ P 〈 0.05 ）. The level of serum TNF-α in low-dose arteannuin group was higher significantly than those in high- dose and media-dose arteannuin group（ P 〈 0. 05 ） , and the level of serum TNF-α in the media-dose group was higher signifi- cantly than that in high-dose arteannuin group（P 〈 0.05 ）. There was no statistically significant difference in the expression of TNF-α mRNA between the media-dose and low-dose arteannuin group （P 〉 0.05 ）. The expression of TNF-α mRNA in high- dose arteannuin group was lower significantly than those in media-dose and low-dose arteannuin group（ P 〈 0.05 ）. The expression of TNF-α protein in high-dose and media-dose arteannuin group were lower significantly than those in control group and low-dose arteannuin group（P 〈0. 05）, but there was no statistically significant difference in the expression of TNF-α protein between media-dose and high-dose arteannuin group （ P 〉 0.05 ）. Conclusion Arteannuin can degrade the level of TNF-α in rats with os- tarthr

关 键 词：青蒿素 骨关节炎 肿瘤坏死因子-Α 大鼠 

分 类 号：R684.3[医药卫生—骨科学]