6个主效基因在苏钟猪分子育种中的应用价值  

Application value of six major genes for molecular breeding of Suzhong swine

作　　者：方晓敏[1] 孟翠[1] 任守文[1] 李碧侠[1] 王学敏[1] 涂枫[1] 付言峰[1] 

机构地区：[1]江苏省农业科学院畜牧研究所,江苏南京210004

出　　处：《江苏农业学报》2012年第5期1074-1078,共5页Jiangsu Journal of Agricultural Sciences

基　　金：江苏省农业科技自主创新基金项目[CX(10)121];江苏省农业科学院学科带头人优秀后备人才项目(6510818);国家生猪现代产业技术体系项目(nycytx-009)

摘　　要：利用PCR-RFLP方法检测了Hal基因、RN基因、ESR基因、FSHβ基因、IGF-1基因及MC4R基因在苏钟猪(180头)、二花脸猪(64头)、梅山猪(60头)、长白猪(46头)、大白猪(48头)和皮特兰猪(35头)猪群中的多态性,探讨了这6个主效基因在苏钟猪分子育种中的应用价值。结果显示:各主效基因在试验猪群中均表现不同程度多态性;其中,在苏钟猪群中Hal基因检测到NN、Nn基因型,RN基因表现为RN-/rn+、rn+/rn+基因型,ESR基因、FSHβ基因、IGF-1基因及MC4R基因分别检测到相关性状优势基因型。这些结果预示各主效基因在苏钟猪分子选育中具有应用价值。The polymorphisms of Hal,RN,ESR,FSHβ,IGF-1 and MC4R genes were detected by PCR-RFLP in Suzhong swine（n=180）,Erhualian swine（n=64）,Meishan swine（n=60）,Landrace swine（n=46）,Large white swine（n=48）,and Pietrain swine（n=35）,and the application value of these genes for molecular breeding in Suzhong swine population was assessed.Polymorphisms were identified for six major genes among the swine breeds.In Suzhong swine population,NN and Nn genotypes of Hal gene,RN-/rn＋ and rn＋/rn＋ genotypes of RN gene were found.Predominant genotypes of ESR,FSHβ,IGF-1 and MC4R genes were observed in the Suzhong swine mentioned above.The results indicate these major genes are useful for molecular breeding of Suzhong swine.

关 键 词：苏钟猪 分子育种 Hal基因 RN基因 ESR基因 FSHΒ基因 IGF-1基因 MC4R基因 

分 类 号：S813.2[农业科学—畜牧学]