糖尿病神经痛大鼠脊髓背角NMDA受体表达的变化  

The changes of expression of NMDA receptors in spinal dorsal horn neurons in rats with diabetic neuropathic pain

作　　者：刘飞飞[1] 马江红[1] 王秀丽[1] 

机构地区：[1]河北医科大学第三医院麻醉科,石家庄市050051

出　　处：《河北医药》2012年第21期3205-3207,共3页Hebei Medical Journal

基　　金：国家自然科学基金( 编号: 30772082);河北省自然科学基金( 编号: C2008000931); 河北省卫生厅资助课题 ( 编号: 07090;20100105)

摘　　要：目的探讨糖尿病神经病理性痛(DNP)大鼠脊髓背角N-甲基-D-天冬氨酸(NMDA)受体表达的变化。方法 SD雄性大鼠24只,4周龄,体重130～150g,随机分为2组,DNP组(D组,n=15)腹腔注射链尿佐菌素(STZ)50mg/kg制备成DNP模型,正常对照组(C组,n=9)给予等容量0.9%氯化钠溶液。给予STZ或0.9%氯化钠溶液后3、5、7周时分别取D组(5只)和C组(3只)大鼠,测量体重,采集静脉血样,测定空腹血糖浓度,然后测定机械缩足阈值,取脊髓组织,采用免疫组织化学法分别测定脊髓背角NR1和NR2B亚单位表达水平。结果与C组比较,在各时间点,D组体重下降,血糖升高,机械缩足阈值明显降低(P<0.05),脊髓背角NR1和NR2B亚单位免疫阳性细胞数表达明显增加(P<0.05)。结论脊髓背角NMDA受体在脊髓水平参与了大鼠糖尿病神经病理性痛的形成。Objective To investigate the changes of expression of NMDA receptors in spinal dorsal horn neurons in rats with diabetic neuropathic pain （DNP）. Methods Twenty-four male SD rats （4-week age,weighed 130- 150g ） were randomly divided into two groups：control group （ n = 9）, DNP group （ n = 15 ）. The DNP models were established by peritoneal injection with streptozotocin （STZ, 50mg/kg ） in DNP group, however, instead of STZ the equal volume of O. 9% sodium chloride solution was injected in control group. At 3,5,7 weeks after the injection the 5 rats in DNP group and 3 rats in control group were taken to be weighed, and the fasting blood glucose levels and paw withdraw threshold （PWT） were measured, and the expression levels of NR1 and NR2B subunits receptors in spinal dorsal horn neurons were detected by immunohistochemistry. Results As compared with those in control group,the rat＇ s body weight in DNP group was decreased, however, the rat＇ s blood glucose was increased, especially, PWT was significantly reduced （ P 〈 0.05 ）, and the expression levels of NR1 and NR2B subunits receptors in spinal dorsal horn neurons were significant increased （ P 〈 0.05）. Conclusion The NMDA receptor in spinal dorsal horn neuron is involved in the pathogenesis of diabetic neuropathic pain in rats.

关 键 词：糖尿病 神经病变 脊髓背角 NMDA受体 NR2B NR1 免疫组织化学 

分 类 号：R587.25[医药卫生—内分泌]