比色法测定血清缺血修饰白蛋白的临床应用评价  

The clinical value of measuring ischeia modified albumin using photocolorimetry

作　　者：孙振荣[1] 潘曙明[2] 戴屹东[1] 赵洁[2] 朱剑骞[1] 林强[2] 周懿忆[1] 王海平[1] 茅以理[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院卢湾分院检验科,200020 [2]上海交通大学医学院附属新华医院急救中心,200092

出　　处：《蚌埠医学院学报》2012年第11期1338-1340,共3页Journal of Bengbu Medical College

摘　　要：目的:评价比色法测定血清缺血修饰白蛋白(IMA)的临床应用价值。方法:采用比色法对46例心肌肌钙蛋白阳性或心电图ST段异常的急诊胸痛患者和70名健康体检者的血清IMA进行了检测,结果作ROC曲线分析和临床诊断特性评价。并与超滤法白蛋白钴结合试验进行比较。结果:精密度试验批内CV为1.3%,批间CV为1.8%。ROC曲线下面积0.995,当cutoff值为75 u/ml时,IMA对急性心肌缺血的敏感度、特异性和准确度分别为97.8%、91.4%及94.0%,阳性预测值、阴性预测值分别为88.2%和98.5%。2种方法测定血清IMA结果差异无统计学意义(P>0.05)。结论:比色法测定血清IMA,实验重复性良好,较超滤法实验方法简便。较高的实验敏感度和阴性预测值显示了IMA作为急性冠状动脉综合征排除诊断或胸痛鉴别诊断指标的临床价值。Objective：To evaluate the clinical value of measuring serum ischemia modified albumin（IMA） using photocolorimetry. Methods：The IMA levels of 46 cases with cTnI positive or electrocardiogram ST session abnormal and 70 healthy subjects were measured through photocolorimetry,analysed by ROC curve,evaluated by the characters of clinical diagnosis and compared with the ultrafiltration albumin cobalt binding test. Results：The intra and inter-assay CV of the precision test results were 1.3% and 1.8%,respectively,and the AUC of ROC curve was 0.995.When the cutoff value was 75 u/ml,the sensitivity,specificity and accuracy of IMA were 97.8%,91.4% and 94.0%,respectively,and the positive and negative predictive value were 88.2% and 98.5%,respectively.The IMA levels detected by two methods had no statistically significance（P0.05）. Conclusions：Compared with the ultrafiltration albumin cobalt binding test,the photocolorimetry to measure the serum IMA is good repeatability and simple and convenient.The high sensitivity and negative predictive value show the high clinical value of IMA for acute coronary syndromes;exclusion diagnosis or thoracalgia diagnosis.

关 键 词：急性冠状动脉综合征 比色法 缺血修饰白蛋白 

分 类 号：R541.4[医药卫生—心血管疾病] R446[医药卫生—内科学]