聚(乙烯基酯树脂-甲基丙烯酸甲酯)整体柱的ATRP法制备及其在蛋白分离中的应用  

Preparation of poly(vinyl carboxylate-co-methyl methacrylate) monolith by ATRP and its application in protein separation

作　　者：白立改[1,2] 杨更亮[1,2] 雷环[1,2] 王洋[1,2] 闫翠红[1,2] 

机构地区：[1]河北省药物质量研究重点实验室,保定071002 [2]河北大学药学院,保定071002

出　　处：《科学通报》2012年第31期2942-2947,共6页Chinese Science Bulletin

基　　金：国家自然科学基金(21175031);河北省自然科学基金(2008416);河北省科技攻关项目(06276479B;07276407D)资助

摘　　要：以乙烯基酯树脂-甲基丙烯酸甲酯为二元单体,通过活性可控自由基聚合方式(原子转移自由基聚合,ATRP)制备了聚(乙烯基酯树脂-甲基丙烯酸甲酯)整体柱.红外光谱测得聚合物表面功能基团,扫描电子显微镜观察了聚合物的内部形态,压汞法考察了聚合物的孔径分布等参数.将该整体柱用作高效液相色谱的固定相,不仅对人血浆中的免疫球蛋白进行了成功分离,并对α-淀粉酶、溶菌酶、蜗牛酶和木瓜酶的混合物进行了成功分离.结果表明,通过活性可控自由基聚合制备得到的整体柱具有结构均匀、通透性好、易于实现聚合物功能化等优点,是一种简单、方便、高效、廉价的聚合物整体柱的制备方法.Poly(vinyl ester resin-co-methyl methacrylate) monoliths have been prepared by atom transfer radical polymerization.Morphology of monolithic materials was studied by scanning electronic microscopy.Chemical groups of the monolith were assayed by infrared spectra method and the pore size distribution was determined by a mercury porosimeter.The monolith was used to separate IgG from human plasma successfully by HPLC.Moreover,the monolith also used to separate the mixture of α-amylasa,lysozyme,snailase and papain.The results showed the prepared monoliths by living/control radical polymerization have uniform structure,good permeability and easy obtained.So the living/control radical polymerization is a simple,convenient,efficient and cheap method for the preparation of polymeric monoliths.

关 键 词：聚合物整体柱 原子转移自由基聚合(ATRP) 高效液相色谱(HPLC) 蛋白分离 

分 类 号：O657.72[理学—分析化学]