胶原性关节炎大鼠血清中TNF-α,IL-17及IL-10水平分析  

Analysis of TNF-α,IL-17 and IL-10 levels in the serum of rats with collagen-induced arthritis

作　　者：宋光[1] 裘红梅[1] 

机构地区：[1]大连大学医学院检验系,辽宁大连116622

出　　处：《大连医科大学学报》2012年第5期492-494,共3页Journal of Dalian Medical University

基　　金：辽宁省高等学校科研项目计划(L2010021)

摘　　要：[目的]检测血清TNF-α,IL-17及IL-10在Ⅱ型胶原诱导性关节炎(CIA)大鼠血清中的表达。[方法]建立CIA动物模型,用酶联免疫吸附实验(ELISA)法检测大鼠模型血清中炎性细胞因子TNF-α、IL-10及IL-17的水平,同时观察大鼠炎症指数。[结果]60只大鼠随机被分为造模组(40只)和对照组(20只),免疫4周后,造模组40只大鼠有32只发病。造模成功组大鼠血清中TNF-α、IL-17水平分别为(39.65±11.23)pg/mL和(46.1±10.9)pg/mL,明显高于造模失败组和正常对照组(P均<0.05),而IL-10水平(29.11±27.59)pg/mL明显低于造模失败组和正常对照组(P<0.05)。另外,造模组大鼠血清TNF-α和IL-17水平与CIA大鼠炎症指数呈正相关关系(r=0.573,r=0.492,P<0.05),与之相反,CIA大鼠血清IL-10水平与炎症指数呈负相关关系(r=-0.481,P<0.05)。[结论]致炎因子(TNF-α,IL-17)和抑炎因子(IL-10)表达失衡可能在CIA发病中具有重要作用。[Objective] To investigate the serum levels of TNF-α,IL-17 and IL-10 in rats with type II collagen-induced arthritis（CIA）.[Methods] The rat model was established using type II collagen and the serum levels of TNF-α,IL-17 and IL-10 were determined by enzyme-linked immunosorbent assay（ELISA）.The arthritis index in CIA rats were observed.[Results] Sixty rats were randomized into two groups：modeling group（40 rats） and normal control（20 rats）.After 4 weeks,the models of CIA were established in 32 rats.The serum levels of TNF-α and IL-17 in the successful modeling group were（39.65±11.23）pg/mL and（46.1±10.9）pg/mL,respectively,significantly higher than that in the abortive modeling group and normal control.While serum concentration of IL-10（29.11±27.59）pg/mL in the successful modeling group was remarkably lower than that in the abortive modeling group and normal control（P0.05）.In addition,There were positive correlations between TNF-α,IL-17 and arthritis index（r=0.573,r=0.492,P0.05）,negative correlations between IL-10 and arthritis index in CIA rats（r=-0.481,P0.05）.[Conclusion] The imbalance of pro-inflammatory cytokine（TNF-α,IL-17） and anti-inflammatory cytokine（IL-10） network may be playing an important role in the pathogenesis of CIA.

关 键 词：胶原性关节炎 TNF-Α IL-17 IL-10 关节炎症指数 

分 类 号：R593.22[医药卫生—内科学]