MICA/B基因多态性与血吸虫病相关性研究  

Research on MICA/B Gene Polymorphism Association with Schistosomiasis Usceptibility

作　　者：龚拯[1] 罗奇志[1] 林琳[1] 汪世平[2] 余平[1] 

机构地区：[1]中南大学基础医学院免疫学系,湖南长沙410078 [2]中南大学基础医学院病原生物学系,湖南长沙410078

出　　处：《激光生物学报》2012年第5期434-440,共7页Acta Laser Biology Sinica

基　　金：国家自然科学基金资助项目(project No:30870135)

摘　　要：本研究采用PCR-SSP与PCR-SBT方法对正常健康对照组与血吸虫病感染组、血吸虫病性重度肝纤维化病人组和轻度肝纤维化病人组中MICA/B基因进行分型,并比较各组基因的多态性。结果在血吸虫感染组与健康对照组中共发现13种MICA等位基因和5种MICA-STR基因型,MICA*012:01(11.58%vs 5.83%)、MI-CA*017(2.11%vs 0.00%)及MICA*027(3.16%vs 0.97%)在对照人群组较血吸虫病人组中分布频率较高,但Pc值显示没有统计学意义(Pc>0.05)。MICA-STR型别分析显示,MICA-STR与血吸虫病易感没有相关性,但MICA*A5基因型的分布频率在重度肝纤维化组显著高于轻度肝纤维化组(45.10%vs 26.92%,Pc<0.05)。在血吸虫病人组中一共检出10种MICB等位基因。在本研究人群中未发现与日本血吸虫感染显著相关的MICB等位基因。同时MICB等位基因多态性在重度纤维化组、轻度纤维化组、以及正常对照组相互之间均无显著的相关性。研究显示在血吸虫病人组中,MICA和MICB具有连锁不平衡,其中单倍型MICB*008-MICA*002:01和MICB*014-MICA*045在血吸虫病人组中显示具有显著的连锁不平衡。In this study, we investigated whether MICA/B polymorphisms were associated with the particularly high rate of Schistosoma parasitic worm infection or severity of disease outcome in the Dongting Lake region of northern Hunan Province, China. MICA/B genotypes were determined by using two methods： PCR-sequence-specific primers （PCR- SSP） and PCR-sequence-based typing （PCR-SBT）. Thirteen MICA alleles and five short-tandem repeat （STR） alleles were identified in the health control and the Dongting Lake area population. The frequency of three alleles, MICA ＊ 012 ： 01（11.58% vs5.83%）, MICA＊017（2.11% vs0.00%） and MICA＊027（3.16% vs0.97%）, were higher in healthy controls than in schistosomiasis patients, but the difference was not significantly correlated with susceptibility to S. japonicum infection （Pc 〉 O. 05 ）. MICA ＊ A5 allele frequency was significantly higher in advanced liver fibrosis groups than slight liver fibrosis groups（ Pc 〈 0. 05 ）. This study provides the initial evidence that MICA genetic polymor-phisms may underlie the severity of liver fibrosis developed in schistosomiasis patients . Ten MICB alleles were identified in the patient group. There were not significant differences in allele frequencies of the MICB gene both between the pa- tients and the control, and between the advanced fibrotic group and the mild fibrotic group. Two kinds of MICA-MICB haplotypes were MICB ＊ 008-MICA ＊ 002：01 and MICB ＊ 014-MICA ＊ 045 in schistosomiasis infection group.

关 键 词：HLA MICA MICB PCR-SBT PCR-SSP 基因多态性 日本血吸虫 

分 类 号：R532.21[医药卫生—内科学]