曲安奈德对瘢痕疙瘩成纤维细胞Ⅰ、Ⅲ型前胶原基因表达的影响  

Assessment of triamcinolone acetonide on the expression of Ⅰ and Ⅲ procollagen genes in human keloid fibroblasts

作　　者：冯红霞[1,2] 辛燕[1,2] 郝玉琴[1,2] 金兰兰[1,2] 

机构地区：[1]内蒙古医科大学第三附属医院 [2]内蒙古包钢医院皮肤科,包头市014010

出　　处：《中国麻风皮肤病杂志》2012年第11期771-775,共5页China Journal of Leprosy and Skin Diseases

摘　　要：目的:评价曲安奈德对瘢痕疙瘩成纤维细胞增殖及Ⅰ、Ⅲ型前胶原mRNA表达的影响。方法:取人瘢痕疙瘩及正常皮肤组织培养的第58代的成纤维细胞,在含有不同浓度的曲安奈德的环境中培养。应用噻唑蓝(MTT)法检测细胞增殖、RT-PCR检测Ⅰ、Ⅲ型前胶原mRNA表达。结果:浓度为0.05～1.0g/L的曲安奈德能明显抑制瘢痕疙瘩和正常皮肤成纤维细胞的增殖;浓度为0.10～1.0g/L的曲安奈德能降低瘢痕疙瘩和正常皮肤成纤维细胞Ⅰ、Ⅲ型前胶原mRNA表达。所有的抑制作用在浓度1g/L时达到最高。结论:曲安奈德对瘢痕疙瘩和正常皮肤成纤维细胞的增殖及Ⅰ、Ⅲ型前胶原mRNA表达有明显的抑制作用。Objective： To assess the impact of triameinolone acetonide on the proliferation and expression of Ⅰ, Ⅲ procollagen mRNA in human keloid fibroblasts. Methods： Triamcinolone acetonide of different concentration was added to cultured fibroblasts for 5 - 8th generations of passage from human keloid and normal skin tissue. Proliferation activity of fibroblasts was examined using MTF and RT - PCR assay to analyze the expression of I and III procollagen mRNA. Results： Proliferation of fibroblast from the keloids and normal skin were inhibited significantly. The concentration of 0.05 - 1.0 g/L triamcinolone acetonide was markedly inhibited the proliferation of fibroblasts and the concentration of 0.10 ～ 1.0 g/L triamcinolone acetonide decreased the expression of I, III procollagen mRNA in fibroblasts cultured from keloid and normal skin. The inhibitive effects were the most high when 1 g/L triamcinolone acetonide added. Conclusion： Triamcinolone acetonide can markedly inhibit the proliferation and the expression of I and III procollagen mRNA of fibroblasts from keloids and normal skin.

关 键 词：瘢痕疙瘩 成纤维细胞 曲安奈德 Ⅰ、Ⅲ型前胶原mRNA 

分 类 号：R622[医药卫生—整形外科]