Curcumin protects against staurosporine toxicity in rat neurons  

作　　者：Xiao-Yan Qin Ji-Hui Lv Jia Cui Xue Fang Yan Zhang 

机构地区：[1]State Key Laboratory of Biomembrane and Membrane Biotechnology, College of Life Sciences, Peking University, Beijing, 100871 Laboratory of Biotechnology and State Key Laboratory of Chinese Ethnic Minority Traditional Medicine, College of Life & Environmental Science, Minzu University of China, Beijing 100081, China [2]Beijing Geriatric Hospital, Beijing 100095, China

出　　处：《Neuroscience Bulletin》2012年第5期606-610,共5页神经科学通报（英文版）

基　　金：supported by the National Basic Research Development Program of China(2009CB941301);the National Natural Science Foundation of China (91132718, 30701138);the Peking University President Research Grant;the Ministry of Education Recruiting Research Grant;the Fundamental Research Funds for the Central Universities of China (1112KYZY41);the "985 Project" of Minzu University, China (MUC98504-14)

摘　　要：Objective Curcumin is extracted from the turmeric plant （Curcuma longa Linn.） and is widely used as a food additive and traditional medicine. The present study investigated the activity of curcumin against staurosporine （STS） toxicity in cell culture. Methods Rat hippocampal neurons in primary culture were exposed to STS （20 μmol/L） and treated with curcumin （20 μmol/L）. Cell viability was tested by MTT assay and reactive oxygen species （ROS） were measured using the MitoSOX red mitochondrial superoxide indicator. Western blot was used to assess changes in the levels of caspase-3 （Csp3）, heat shock protein 70 （Hsp70） and Akt. Results The results showed that curcumin protects against STS-induced cytotoxicity in rat hippocampal neurons. Csp3, Hsp70, Akt and ROS activation may be involved in this protection. Conclusion Curcumin could be a potential drug for combination with STS in cancer treatment to reduce the unwanted cytotoxicity of STS.Objective Curcumin is extracted from the turmeric plant （Curcuma longa Linn.） and is widely used as a food additive and traditional medicine. The present study investigated the activity of curcumin against staurosporine （STS） toxicity in cell culture. Methods Rat hippocampal neurons in primary culture were exposed to STS （20 μmol/L） and treated with curcumin （20 μmol/L）. Cell viability was tested by MTT assay and reactive oxygen species （ROS） were measured using the MitoSOX red mitochondrial superoxide indicator. Western blot was used to assess changes in the levels of caspase-3 （Csp3）, heat shock protein 70 （Hsp70） and Akt. Results The results showed that curcumin protects against STS-induced cytotoxicity in rat hippocampal neurons. Csp3, Hsp70, Akt and ROS activation may be involved in this protection. Conclusion Curcumin could be a potential drug for combination with STS in cancer treatment to reduce the unwanted cytotoxicity of STS.

关 键 词：CURCUMIN staurosporine-induced cytotoxicity hippocampal neurons caspase-3 Akt Hsp70 reactive oxygen species cancer treatment 

分 类 号：Q426[生物学—神经生物学] TQ463.4[生物学—生理学]