UVA联合UVB照射对原代人角质形成细胞增殖的影响  

Effects of ultraviolet A and ultraviolet B irradiation on the proliferation of primary human keratinocytes

作　　者：张丽[2] 吴文娟[1] 起珏[1] 涂颖[1] 顾华[1] 何黎[1] 

机构地区：[1]昆明医科大学第一附属医院皮肤科,昆明650032 [2]昆明市第一人民医院皮肤科,昆明市650011

出　　处：《中华皮肤科杂志》2012年第11期825-827,共3页Chinese Journal of Dermatology

基　　金：云南省科技强省计划,应用基础研究重点项目（2007C0011Z）

摘　　要：目的探讨95％长波紫外线（UVA）联合5％中波紫外线（UVB）照射对原代人表皮角质形成细胞（HEK）增殖的影响，为构建13光对人工皮肤光损伤模型提供实验依据。方法用总剂量分别为0、2．5、5、10、20、30、40、60J／cm^2的紫外线（含95％UVA和5％UVB），分别照射体外培养的HEK，继续培养24h后，用噻唑蓝（MTT）法检测细胞活力，用SPSS软件计算引起HEK半数损伤时的紫外线照射剂量。结果8种剂量紫外线照射后，HEK相对抑制率分别为0、1．03％、6．60％、17．28％、31．28％、49．59％、59．67％、70．99％，当总照射剂量≥10J／cm^2时，HEK相对抑制率与对照组相比，差异有统计学意义（F=62．11，P〈0．05）；HEK相对抑制率为50％时的紫外线照射总剂量为31．31J／cm^2。结论随着紫外线照射剂量的加大，HEK增殖活力降低，31．31J／cm^2为HEK半数死亡的照射剂量。Objective To observe the effect of uhraviolet irradiation eomparising 95% ultraviolet A （UVA） and 5% ultraviolet B （UVB） on the proliferation of human epidermal keratinoeytes （HEKs）, in hope to offer a basis for the construction of a photodamaged skin model indueed by sunlight. Methods HEKs were isolated from foreskin tissue and cuhured in vitro. After several passages, the HEKs were irradiated with different doses （0, 2.5, 5, 10, 20, 30, 40, 60 J/cm^2 ） of UV comprising 95% UVA and 5% UVB. Methyl thiazolyl tetrazolium （MTI＇） assay was used to evaluate cell viability after 24 bours of additional euhure. SPSS 17.0 software was used to calculate the median lethal close （LD50） of uhraviulet radiation in HEKs. Results Tbe proliferation of HEKs was inhibited by 0, 1.03%, 6.60%, 17.28%, 31.28%, 49.59%, 59.67% and 70.99% respectively after irradiation with UV of 0, 2.5, 5, 10, 20, 30, 40 and 60 J/cm^2 . A significant inhihition of cell proliferation was observed in HEKs irradiated with UV at a close of no lower than 10 J/cm2 eompared with unilTadiated HEKs （F = 62.11, P 〈 0.05）. The LD50 of UV in HEKs was 31.31 J/cm^2 . Conclusions Aas the dose of UV irradiation increases, the proliferative activity of HEKs decreases, with the LD50 of UV being 31.31 J/cm^2 .

关 键 词：角质形成细胞增殖 UVB照射 UVA 人表皮角质形成细胞 中波紫外线 照射剂量 紫外线照射 SPSS软件 

分 类 号：R751[医药卫生—皮肤病学与性病学]