结构型热休克蛋白70在类风湿关节炎患者滑膜组织和血液中表达的研究  

The expression of Hsc70 in synovial membranes and blood of patients with rheumatoid arthritis

作　　者：孟庆松[1] 赵燕[5] 孙水[6] 常晓天[2] 刘文波[3] 闫新峰[4] 

机构地区：[1]山东济南大学附属山东省千佛山医院检验科,山东济南250014 [2]山东济南大学附属山东省千佛山医院医学实验中心,山东济南250014 [3]山东济南大学附属山东省千佛山医院风湿免疫科,山东济南250014 [4]山东济南大学附属山东省千佛山医院骨关节科,山东济南250014 [5]山东省医药生物技术研究中心 [6]山东大学附属省立医院骨关节科

出　　处：《中华风湿病学杂志》2012年第11期741-744,I0001,共5页Chinese Journal of Rheumatology

基　　金：山东省自然科学基金（Y2007C160）

摘　　要：目的在蛋白和mRNA水平上研究结构型热休克蛋白70（Hsc70）在类风湿关节炎（RA）患者滑膜和血液中的表达水平，探讨该蛋白对RA的病理作用。方法用免疫组织化学法、实时荧光定量聚合酶链反应、蛋白印迹法分析Hsc70在RA滑膜中的表达水平；以健康人为对照，用酶联免疫吸附法分析Hsc70在RA患者血液中的表达水平。采用单因素方差分析、LSD检验、Spearman相关分析进行统计学处理。结果与骨关节炎和强直性脊柱炎比较，Hsc70在RA滑膜高表达，表达率为100％。Hsc70在RA血液中表达量（吸光度值O．32±0．09）也高于健康组（吸光度值0．16±0．03）的表达量（P〈0．01）。结论Hsc70在RA滑膜组织中高表达，提示Hsc70在RA发病过程中可能起重要作用。Objective The present study investigated the expression of heat shock cognate protein 70 （Hse70） in the synovial tissues and blood samples of patients with rheumatoid arthritis （RA） to determine the pathological role of this protein in the pathogenesis of the disease. Methods The expression of Hsc70 in synovial membranes was quantitatively analyzed by immunohistochemistry, real-time quantitative PCR and western blotting. The samples from osteoarthritis （OA） and ankylosing spondylitis （AS） were used as controls. The levels of Hsc70 in blood of patients with RA were determined using enzyme linked immunosorbent assay （ELISA） with the samples of the healthy subjects as controls. Statistical analysis was conducted with one-way ANOVA, LSD test and Spearmen＇s correlation. Results Immunohistochemistry showed that Hsc70 had significantly increased expression in synovial tissues of RA than in the samples of OA and AS. Real-time PCR and western blotting confirmed the above findings. ELISA detected significantly elevated level of Hsc70 in blood of patients with RA as compared with samples from the controls （P〈0.01）. Conclusion The study suggests that the up-regulation of Hsc70 may be involved in the pathogenic process of RA.

关 键 词：关节炎 类风湿 热休克蛋白质70 免疫测定 

分 类 号：R593.22[医药卫生—内科学]