黄芪注射液对急性肺损伤小鼠钠通道表达的影响  

Effect of astragali injection on epithelial sodium channel expression in mice with acute lung injury

作　　者：赵燕[1] 何婧[1] 管弦[1] 王导新[1] 

机构地区：[1]重庆医科大学附属第二医院呼吸内科,重庆400010

出　　处：《南方医科大学学报》2012年第11期1603-1605,1609,共4页Journal of Southern Medical University

基　　金：国家自然科学基金(30971303)~~

摘　　要：目的探讨黄芪注射液对急性肺损伤(ALI)小鼠钠通道表达的影响及其可能的机制。方法 30只C57BL/6小鼠随机分为3组,对照组(NS组)、模型组(ALI组)和黄芪注射液组。观察各组12 h后肺干湿质量比、支气管肺泡灌洗液(BALF)中炎症细胞比例变化,并采用HE染色观察肺组织病理改变,Q-PCR检测α-ENaC、TNF-α、IL-8的mRNA表达水平。结果 ALI组BALF中性粒细胞比例升高,与其他2组比较差异有统计学意义。病检示黄芪注射液组肺组织炎症、充血水肿等情况有明显减轻。与NS组相比,ALI组α-ENaC mRNA的表达显著降低,TNF-α、IL-8mRNA表达显著升高。而黄芪注射液组的α-ENaC mRNA的表达显著高于ALI组,TNF-α、IL-8mRNA表达位于3组居中水平。结论黄芪注射液可通过抑制急性肺损伤时炎症介质水平,上调ENaC表达,促进肺水肿液清除,减轻肺组织损伤。Objective To investigate the effect of astragali injection on the expression of epithelial sodium channel in mice with acute lung injury （ALI） and explore the possible mechanism. Methods Thirty C57BL/6 mice were randomized into 3 equal groups, namely the control group, ALI model group, and astragali injection treatment group. Twelve hours after the treatments, The wet-dry ratio （W/D） of the lungs, inflammation cell percentages in the bronchoalveolar lavage fluid （BALF） and histopathological changes of the lung tissues were examined, and the expressions of a-ENaC, TNF-a, and IL-8 mRNA in the lung tissues were determined with quantitative RT-PCR. Results The neutrophil percentage in the BALF increased significantly in ALl group as compared with that in the other two groups. Pathological examination revealed milder lung tissue inflammation, congestion and edema in astragalus injection treatment group than in the ALI model group. Compared with those in the control group, a-ENaC mRNA expression decreased significantly while TNF-a and IL-8 mRNAs increased markedly in ALI group. In astragalus injection treatment group, the expression level of a-ENaC mRNA was higher than that in ALI group, and TNF-a and IL-8 mRNA expression lower than those in ALI group but higher than those in the control group. Conclusion Astragalus injection can ameliorate ALI in mice by inhibiting the release of inflammatory factors and up-regulating ENaC mRNA expression to promote the clearance of pulmonary edema fluid.

关 键 词：急性肺损伤 黄芪注射液 炎症 钠离子通道 

分 类 号：R285.5[医药卫生—中药学]