手参组织培养和植株再生技术研究  

Tissue Culture and Plant Regeneration of Gymnadenia conopsea

作　　者：高晓玲[1] 冯鸿[1] 

机构地区：[1]四川教育学院,四川成都610041

出　　处：《西南农业学报》2012年第5期1841-1844,共4页Southwest China Journal of Agricultural Sciences

基　　金：四川省科技厅应用基础研究项目(2010JY0039)

摘　　要：对手参组织培养和植株再生的技术条件和参数进行了研究。结果表明,接近成熟的4月胚龄的种胚的萌发和成苗最具优势。PT培养基为手参胚萌发的最适培养基,在其中加入10%的马铃薯提取物可提高萌发率和成苗率,加1%的活性炭可对试管苗的生长起到促进作用。在MS培养基中加入0.4 mg/L的6-BA和0.2 mg/L的NAA,试管苗茎尖的增殖效果最佳。生根培养时在MS培养基中加0.5 mg/L NAA和0.6 mg/L IBA的效果最好,加1%的活性炭有利于生根壮苗。The tissue culture and plant regeneration of Gymnadenia conopsea were studied.The results indicated that the 4 month embyro culture had the highest sprouting and plantlets capability.The optimal medium for the embryo culture was PT.Adding 10 % patato extract could promote embyros sprouting and plantlets formation,1 % active carbon could improve plantlets growing.MS ＋ 6-BA 0.4 mg/L ＋ NAA 0.2 mg/L had the best effectiveness for the stem tip proliferation.The best medium of rooting was MS ＋ NAA 0.5 mg/L ＋ 0.6 mg/L IBA,and 1 % active carbon could improve rooting.

关 键 词：手参 胚 组织培养 生根 

分 类 号：S567.59[农业科学—中草药栽培]