TMB-8体外对神经细胞保护作用及其机理的研究  

Protective Effects and Mechanism of TMB 8 on Neuron

作　　者：徐昌盛[1] 王斌[1] 肖继皋[1] 

机构地区：[1]南京医科大学药理学教研室,南京210029

出　　处：《南京医科大学学报（自然科学版）》2000年第3期192-194,共3页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省教委自然科学基金!资助项目 ( JW970 0 96

摘　　要：目的　研究 TMB- 8[8- (N,N-二乙胺 ) - n-辛基 - 3,4,5 -三甲氧基苯甲酸酯 ]的神经保护作用及其可能机制。方法　用氧 -葡萄糖剥夺模型观察 TMB- 8对培养的大鼠神经细胞形态、细胞膜通透性和细胞内丙二醛 (MDA)、总过氧化物歧化酶 (T-SOD)、谷胱甘肽 (GSH)和谷胱甘肽过氧化物酶 (GSH- PX)的影响。结果　经氧 -葡萄糖剥夺 1h后 ,无血清培养 2 4h的神经细胞乳酸脱氢酶 (L DH)漏出明显增加 ,细胞内 GSH的含量和 T- SOD的活性显著降低 ,TMB- 8能剂量依赖性地减少 L DH的漏出 ,能升高细胞内 GSH和提高细胞内 T- SOD的活性。结论　 TMB- 8的神经保护作用可能与增加神经细胞内 GSH的水平和提高 T-SOD的活性有关。Objective To investigate the neuroprotective effect and mechanism of TMB 8[8 (N, N diethylamino) n octyl 3,4,5 trimethoxybenzoate].Methods The oxygen glucose deprivation model of cultured rat neuron was used to observe the effects of TMB 8 on neuron morphology, permeability and contents of MDA, GSH, T SOD and GSH px in neurons.Results The damaged neurons induced by oxygen glucose deprivation for 1 h and after serum free culture for 24 h could liberate much LDH; the contents of GSH and the activity of T SOD in neurons were significantly decreased; TMB 8 could concentration dependently reduce the efflux of LDH from the damaged neuron and increase the content of GSH in neuron, the activity of T SOD in these cells was also increased.Conclusion the neuroprotective role of TMB 8 may be related to the increasing of GSH and T SOD in the damaged neuron.

关 键 词：三甲氧基苯甲酸酯 神经细胞 GSH SOD TMB-8 

分 类 号：R338[医药卫生—人体生理学]