甜叶菊SRAP分子技术优化及亲缘关系分析  

Optimization of SRAP Molecular Technology and Analysis of Genetic Relationship in Stevia rebaudiana Bertoni

作　　者：胡能兵[1,2] 何克勤[1,2] 张子学[1,2] 林平[1,2] 隋益虎[1,2] 周玉丽[1,2] 崔广荣[1,2] 

机构地区：[1]安徽科技学院植物科学学院,安徽凤阳233100 [2]安徽省甜叶菊工程技术研究中心,安徽蚌埠233000

出　　处：《安徽科技学院学报》2012年第5期15-22,共8页Journal of Anhui Science and Technology University

基　　金：安徽省教育厅省级重点自然科学基金项目(KJ2010A073);蚌埠市科技计划项目(科字051);安徽科技学院青年基金项目(ZRC2011281)

摘　　要：以甜叶菊为试材,采用正交法对影响其相关序列扩增多态性(SRAP)反应体系的5个因子进行优化,进而对来自国内外的12个品种进行亲缘关系分析。结果表明,5个因素对SRAP反应体系影响大小顺序为:MgCl2,Primers,Taq,dNTPS和DNA。20μL的SRAP优化体系包括:双蒸水12.7μL,10×Buffer(含15mmol/L MgCl2)溶液3μL,2.5 mmol/L的dNTPS 0.8μL,10μmol/L的前后引物各0.75μL,20ng的模板DNA 1μL,5U Taq聚合酶;品种内遗传距离的变异范围为0.24～0.69,当遗传距离为0.48时,可将上述12个甜叶菊品种分为四类。Optimization of 5 factors of sequence-related amplified polymorphism（SRAP） reaction system using the orthogonal design and relationship analysis of 12 kinds of cultivars in Stevia rebaudiana Bertoni were studied.The results indicated that the factor influencing SRAP system followed the order： MgCl2,Primers,Taq,dNTPS and DNA.The optimization system of 20μL included 12.7μL of ddH20,3μL of 10×Buffer with 15mmol/L MgCl2,0.8μL of 2.5 mmol/L dNTPS,0.75μL of 10μmol/L Primers,1μL of 20ng DNA and 5 unit of Taq polymerase.The result of clustering showed that the genetic distance among 12 cultivars ranged from 0.24 to 0.69,and could be classified into four groups when the genetic distance was 0.48.

关 键 词：甜叶菊 相关序列扩增多态性 优化 亲缘关系 

分 类 号：S566.9[农业科学—作物学]