凤尾草多糖两种测定方法的比较  

Comparison of Two Determination Methods for Polysaccharide Content in Pteris multifida Poir

作　　者：周国梁[1] 鲍斌[2] 俞浩[1] 张孝林[1] 刘汉珍[1] 时维静[1] 

机构地区：[1]安徽科技学院食品药品学院,安徽凤阳233100 [2]上海中医药大学药学院,上海200032

出　　处：《安徽科技学院学报》2012年第5期38-42,共5页Journal of Anhui Science and Technology University

基　　金：国家科技部科技人员服务企业行动项目(2009GJC30034);安徽省教育厅研究计划项目(KJ2010B301);安徽科技学院青年基金研究计划项目(ZRC2011283)

摘　　要：目的:为选出一种比较准确、方便测定凤尾草多糖的方法。方法:通过热浸法提取凤尾草中多糖,采用蒽酮-硫酸法、苯酚-硫酸法两种方法进行含量测定比较。结果:蒽酮-硫酸法和苯酚-硫酸法的稳定性实验RSD分别为1.56%和0.68%,精密度实验RSD分别为9.35%和2.28%,回收率平均值分别91.71%和99.23%。结论:苯酚-硫酸法具有稳定性好、精密度高,而且回收率实验也较为理想,是测定凤尾草多糖含量较为理想的方法。Objective： In order to select an accurate,convenient method of Pteris multifida Poir polysaccharide determination.Methods： This experiment by hot leaching extracted polysaccharide,the methods of Phenol-sulfuric acid method and Anthrone-sulfuric acid method were compared.Results： The results showed the RSD value of the two methods were 1.56% and 0.68% in stability experiment,9.35% and 2.28% in accuracy experiment,the average recovery was 91.71 % and 99.23% respectively.Conclusion： The experimental design of Phenol-sulfuric acid method is simple,reliable and highly predictive and the result of recovery experiment was more satisfactory.So the better method to determine Pteris multifida polysaccharide content was Phenol-sulfuric acid method.

关 键 词：凤尾草 多糖含量 苯酚-硫酸法 蒽酮-硫酸法 紫外分光光度法 

分 类 号：R284[医药卫生—中药学]