副猪嗜血杆菌外膜蛋白表型分析  

Analysis of outer membrane proteins phenotype of Haemophilus parasuis

作　　者：朱必凤[1] 杨旭夫[1] 彭凌[1] 韦昭玉[2] 

机构地区：[1]韶关学院英东生命科学学院,广东韶关512005 [2]南昌大学生命科学学院,江西南昌330047

出　　处：《中国兽医学报》2012年第11期1656-1661,1668,共7页Chinese Journal of Veterinary Science

基　　金：香港霍英东铭源基金(HK314-14591);广东省农业攻关项目(2004B20201019);韶关市科技创新项目(2010140473)

摘　　要：采用SDS-PAGE测定了82个副猪嗜血杆菌分离株细胞外膜蛋白(OMP),比较不同临床背景分离株的OMP表型差异,分析了OMP与毒力菌株的相关性。结果表明,外膜蛋白分为3种类型:PAGE I、PAGE II和PAGEⅢ型。约34%患病猪分离株属于PAGE I型,只有约9%健康猪分离株属于PAGE I型,说明36 000～40 000蛋白与菌株的毒力相关。The out membrane protein （OMP） in 82 isolations of Haemophilus Parasuis were deter- mined by SDS-PAGE. We compared the diversity of the OMP phenotype of isolates from diffent clinical background to explore the associativity between OMP and virulence pathogen. The results showed that isolates are divided into 3 major PAGE type groups as differece of Rf. PAGE Ⅰ is char- acterized betwee 36 000 and 40 000 OMP,and included about 34% isolates recovered from disease pigs and 9% isolates recovered from healthy pigs; PAGE Ⅱ is characterized by absence of the 36 000-40 000 proteins. It is shown that the 36 000-40 000 OMP could be related to virulence.

关 键 词：副猪嗜血杆菌 外膜蛋白 毒力菌株 

分 类 号：S852.612[农业科学—基础兽医学]