东北地区部分犊牛感染贾第虫的基因型及基因亚型分析  

Genotype in dentification of Giardia from cattle in part areas of northeast China

作　　者：张静[1] 苏艳[1] 白光彦 王春仁[3] 高依然[1] 韩福松[1] 姜宁[1] 陈启军[1] 尹继刚[1] 

机构地区：[1]吉林大学人兽共患病研究所,人兽共患病研究教育部重点实验室,吉林长春130062 [2]吉林市丰满区小白山乡兽医站,吉林吉林市132108 [3]黑龙江八一农垦大学动物科技学院,黑龙江大庆163319

出　　处：《中国兽医学报》2012年第11期1679-1682,1707,共5页Chinese Journal of Veterinary Science

基　　金：国家"十一.五"传染病重大专项资助项目(ZX10004-011)

摘　　要：采用巢式PCR方法鉴定东北部分地区牛源贾第虫的基因型。提取DNA后经巢式PCR扩增TPI基因,扩增产物测序后用BLAST和MEGA 4.0软件进行同源性和系统发育分析。结果表明,吉林市和长春分离株属于蓝氏贾第虫的Assemblage E型;大庆分离株中蓝氏贾第虫的Assemblage E和Assemblage A型均存在。Giardia isolated from feces of naturally infected calves in part areas of northeast China were genotyped by nested PCR. After the genornic DNA of Giardia was extracted, the TPI gene was amplified by nested PCR. The BLAST and MEGA4. 0 were used to analyze their homology and phylogeny. As demonstrated by TPI gene analysis,the Jilin and Changchun isolates belong to Assemblage E of G. larnblia; Assemblage E and A of G. larnblia all existed in Daqing isolates.

关 键 词：贾第虫 基因型 基因亚型 

分 类 号：S852.7[农业科学—基础兽医学]