紫花地丁黄酮类提取物体外抗传染性支气管炎病毒作用研究  

The in vitro antiviral activity of the flavonoids extract from the Chinese medicinalherb Viola yedoensis

作　　者：伍小波[1] 古淑英[1] 罗先钦[2] 罗忠亮[1] 

机构地区：[1]西南大学药学院,重庆400715 [2]重庆市中药研究院,重庆400065

出　　处：《中国兽医学报》2012年第11期1694-1697,共4页Chinese Journal of Veterinary Science

基　　金：西南大学青年基金资助项目(SWU209011);重庆市科技创新能力建设基金资助项目(2009CB1010)

摘　　要：为了在体外研究紫花地丁黄酮类提取物抗鸡传染性支气管炎病毒(IBV-M41)的作用,以鸡胚肾细胞为宿主细胞,阿昔洛韦为阳性对照药物,通过观察细胞病变效应(CPE)与空斑减数试验测定紫花地丁黄酮类提取物抗IBV活性,计算其IC50与治疗指数,并从药物对病毒的直接灭活作用、对病毒吸附的影响及对病毒穿膜的影响3个方面初探紫花地丁黄酮类提取物抗IBV活性的机理。结果:紫花地丁黄酮类提取物能明显抑制IBV的致病变作用,其IC50为16.52mg/L,TI值为15.76。研究显示,紫花地丁黄酮类提取物在体外对IBV直接灭活的效果明显,在高浓度时对抑制IBV吸附与穿入细胞具有一定作用。结果表明:紫花地丁黄酮类提取物在体外有明显的抗IBV感染作用,且主要是通过直接灭活IBV而发挥作用。The antiviral activity of an extract from the Chinese medicinal herb Viola yedoensis has been investigated in this experiment. The flavonoids extract was tested for its antiviral activity a- gainst infectious bronchitis virus （IBV） via cytopathic effect reduction assay and plaque reduction assay in cell culture. The initial studies on antiviral mechanism consisted of three aspects： virucidal assay,adsorption assay and penetration assay, respectively. The flavonoids extract presented a sig- nificant antiviral activity against IBV and had a low cytotoxicity towards chicken embryo kidney （CEK） cells. The 50% inhibitory concentration （IC50） was 16.52 mg/L （TI=15. 76）. At a high concentration of the flavonoids extract （100 mg/L） ,the quantity of plague of IBV was reduced ap- proximately by 90%. The flavonoids extract was able to inactivate virus directly. It is concluded that the flavonoids extract from the Chinese medicinal herb Viola yedoensis has an anti-IBV activi- ty by inactivating virus directly.

关 键 词：紫花地丁 黄酮 鸡传染性支气管炎病毒 细胞病变效应法(CPE) 空斑减数试验 

分 类 号：S852.65[农业科学—基础兽医学]