稀释液成分对猪精液碱性N-乙酰-β-D-氨基葡萄糖苷酶的影响  

Effects of diluter ingredient on the alkaline N-acetyl-β-D-glucosaminidase from porcine semen

作　　者：林心宇[1] 胡崇伟[1] 章文[1] 翁丽蓉[1] 罗忠宝[1] 黄一帆[1] 黄小红[1] 

机构地区：[1]福建农林大学动物科学学院中西兽医结合与动物保健福建省高校重点实验室,福建福州350002

出　　处：《中国兽医学报》2012年第11期1730-1734,共5页Chinese Journal of Veterinary Science

基　　金：福建省自然科学基金资助项目(2010J01059)

摘　　要：以猪精液稀释液主要成分为效应物,研究其对杜洛克猪精液中N-乙酰-β-D-氨基葡萄糖苷酶Ⅱ活力的影响。结果表明:在一定浓度下青霉素钾和硫酸链霉素对酶活力没有影响;EDTA.2Na和维生素C对酶活力有显著的激活作用;葡萄糖和蔗糖对酶活力有先扬后抑的作用;果糖、柠檬酸三钠、三羟甲基氨基甲烷、碳酸氢钠对酶活力则有不同程度的抑制作用;三羟甲基氨基甲烷表现为可逆的非竞争性抑制,抑制常数KI为9.81mmol/L;碳酸氢钠则表现为可逆的混合型抑制,抑制常数KI和KIS分别为111.18mmol/L和26.61mmol/L。The effects of main ingredients in semen diluter on N-acetyl-β-D-glucosaminidaseⅡ （NAGase Ⅱ ） from porcine semen were investigated. The results indicated that penicillin potassium and streptomycin sulphate had no effects on the activity of alkaline NAGase. EDTA. 2Na and Vita- min C showed significantly activating effects on the enzyme activity. The lower concentrations glu- cose and sucrose activated the enzyme, while the higher concentrations inhibited the enzyme activi- ty in some degree. Fructose, trisodium citrate,Tris and sodium bicarbonate showed various degrees of inhibitory effects on the enzyme activity. Tris was a reversible non-competitive inhibitor, with inhibition constants of free enzyme（Kr） was 9.81 mmol/L. Sodium bicarbonate was a mixed-type inhibitor. The inhibition constants of free enzyme （K_I） and enzyme-substrate complex （K_IS） with sodium bicarbonate were determined to be 111.18 mmol/L and 26.61 mmol/L,respectively.

关 键 词：N-乙酰-Β-D-氨基葡萄糖苷酶 猪精液 稀释液成分 

分 类 号：S828.32[农业科学—畜牧学]