亲水作用色谱-蒸发光散射检测器联用法同时测定巴戟天中5种低聚糖的含量  

HILIC-ELSD determination of five oligosaccharides contained in Morinda officinalis

作　　者：邓少东[1] 肖凤霞[1] 林励[1] 张鹏[1] 林靖然[1] 张盛滨[1] 

机构地区：[1]广州中医药大学中药学院,广东广州510006

出　　处：《中国中药杂志》2012年第22期3446-3450,共5页China Journal of Chinese Materia Medica

基　　金：海南省重点科技计划项目(090603);广东省大学生创新实验项目(1057212041)

摘　　要：目的:采用亲水作用色谱-蒸发光散射检测器联用法同时测定巴戟天中蔗糖、蔗果三糖、耐斯糖、1F-果呋喃糖基耐斯糖、巴戟甲素5种低聚糖的含量。方法:采用Waters XBridgeTMAmide(4.6 mm×150 mm,3.5μm)亲水性作用色谱柱;以乙腈(A)-0.2%三乙胺溶液(B)为流动相,梯度洗脱;流速0.8 mL·min-1;柱温40℃;以Waters 2424 ELSD检测,漂移管温度90℃,气压275.79 kPa。结果:5种低聚糖分别在2.128～21.28μg(r=0.999 3),1.864～18.64μg(r=0.999 6),1.92～19.2μg(r=0.999 8),1.912～19.12μg(r=0.999 5),2.368～23.68μg(r=0.999 4)线性关系良好,其回收率在92.81%～102.8%(n=6)。结论:该方法简单、准确,具有良好的重复性,为有效评价巴戟天药材的质量提供了分析方法。Objective： To determine determinate five oligosaccbarides, namely sucrose, 1-kestose, nystose, 1F-fruetofurano- syinystose, bajijiasu contained in Morinda officinalis with an HILIC-ELSD method. Method： Waters XBridgeTM Amide （4. 6 mm × 150 ram, 3.5 μm） hilic column was adopted for gradient elution, with acetonitrile （A） and 0. 2% triethylamine （B） as the mobile phase. The column temperature was set at 40 ℃ , with the flow rate of 0. 8 mL · min^-1. Waters 2424 evaporative light scattering detector （ESLD） was used as detector, with the gas flow of 275.79 kPa and drift tube temperature of 90 μ. Result： The detection range for the tire oligosaccharides were 2. 128-21.28 μg for sucrose （r = 0. 999 3） , 1. 864-18.64 μg for 1-kestose （ r = 0. 999 6） , 1.92-19.2 μg for nystose （r =0. 999 8）, 1. 912-19. 12 μg for 1F-fruetofuranosyinystose （r =0. 999 5）, 2. 368-23.68 μg for bajijiasu （r =0. 999 4 ） , respectively. The recovery of the five oligosaecharides ranged between 92.81% -102. 8% （n = 6 ）. Conclusion： The method is so simple, accurate and highly reproducible that it can be used as an analytical method for effective evaluation of the quality of M. offici- nalis herbs.

关 键 词：巴戟天 亲水作用色谱 蒸发光散射检测器 蔗糖 蔗果三糖 耐斯糖 1F-果呋喃糖基耐斯糖 巴戟甲素 

分 类 号：R284.1[医药卫生—中药学]