6种开心散类方对不同物质损伤神经细胞的保护作用  

Protective effect of six Kaixin San formulas on nerve cells injured by different materials

作　　者：赵海霞[1,2] 周小江[1] 胡园[1] 董宪喆[1] 曹寅[3] 刘屏[1] 

机构地区：[1]解放军总医院临床药理研究室,北京100853 [2]天津中医药大学,天津300193 [3]蚌埠医学院,安徽蚌埠233000

出　　处：《中国中药杂志》2012年第22期3472-3476,共5页China Journal of Chinese Materia Medica

基　　金：国家"重大新药创制"科技重大专项(2008ZXJ09004-028);国家自然科学基金项目(81173430)

摘　　要：目的:研究组方相同,比例不同的6种开心散类方对模拟抑郁、阿尔茨海默病和帕金森病细胞模型的保护作用。方法:以0.4 mmol·L-1皮质酮损伤SH-SY5Y细胞,20μmol·L-1β-淀粉样肽(Aβ25-35)损伤PC12细胞,250μmol·L-11-甲基-4-苯基吡啶离子(1-methyl-4-phenylpyridinium,MPP+)损伤SH-SY5Y细胞,建立体外模拟抑郁、阿尔茨海默病及帕金森模型,采用MTT法检测细胞的存活率及LDH法检测细胞损伤程度。结果:100,500 mg·L-1《备急千金要方》之定志小丸可显著提高皮质酮损伤的SH-SY5Y细胞的存活率并明显减少LDH的释放浓度;各开心散类方均有明显增加Aβ25-35损伤的PC12细胞的存活率、减少LDH的释放浓度的作用;其中以10,100,500 mg·L-1《备急千金要方》之开心散的作用最为显著;500 mg·L-1《古今录验》之茯神丸可显著提高MPP+损伤的SH-SY5Y细胞的存活率、减少LDH的释放。结论:定志小丸对皮质酮损伤的神经细胞有明显的保护作用,有潜在抗抑郁作用;6种开心散类方对Aβ25-35损伤的PC12细胞均有保护作用,有潜在抗阿尔茨海默病作用,《备急千金要方》之开心散作用最为显著;《古今录验》之茯神丸高剂量组对MPP+损伤的SH-SY5Y细胞有一定的保护作用。Objective： To investigate the protective effect of six Kaixin San formulas on simulated cells model of depression, Alzheimer＇s disease and Parkinson＇s disease. Method： The in vitro simulated cells model of depression, Alzheimer＇s disease and Parkinson＇s disease was established by injuring SH-SYSY cells with corticosterone （0.4 mmol ·L^-1 ） , injuring PC12 cells with neurotox- ic amyloid peptide （Aβ25-35） （20 μ mol·L^-1） and injuring SH-SYSY cells with 1-methyl-4-phenylpyridinium ion （MPP ＋ ） （250 μ mol·L^-1）. The cell survival rate was assayed with MTY method and the degree of cell injury was detected with LDH. Result： 100, 500 mg·L^-1 Dingzhixiao Wan prepared as mentioned in Beiji Qianjin Yaofang could significantly increase the survival ratio of SH-SY5Y cells injured by corticosterone and reduce LDH concentration released. All of the Kaixin San formulas could significantly increase the survival ratio of PC12 cells injured by Aβ25-35 and reduce LDH concentration released. Particularly, Kaixin San（ 10, 100, 500 mg·L^-1） prepared as mentioned in Beiji Qianjin Yaofang shown the best effect. And 500 mg·L^-1 Fushen Wan prepared as mentioned in Gujin Luyan could significantly increase survival ratio of SH-SY5Y cell injured by MPP ＋ and reduce LDH concentration released. Conclusion： Dingzhixiao Wan prepared as mentioned in Beiji Qianjin Yaofang could protect corticosterone-induced SH-SYSYcells injury, showing a potential antidepressant effect. All of the six Kaixin San formulas could protect Aβ25-35- induced PC12 ceils injury, but Kaix- in San prepared as mentioned in Beiji Qianjin Yaofang shown the best potential effect for Alzheimer＇s disease. Fushen Wan prepared as mentioned in Gujin Luyan could protect MPP ＋ -induced SH-SY5Y cells injury to some extent.

关 键 词：开心散 SH-SY5Y PC12 抑郁 阿尔茨海默病 

分 类 号：R285[医药卫生—中药学]