不同类型精子发生障碍与精子TEKT5基因mRNA的表达相关性研究  

Study on the correlation between different types of spermatogenesis failure and expression of sperm TEKT5 mRNA

作　　者：江莉[1] 李慕军[1] 覃莉[1] 

机构地区：[1]广西医科大学第一附属医院生殖中心,广西南宁530021

出　　处：《中国妇幼保健》2012年第32期5140-5143,共4页Maternal and Child Health Care of China

基　　金：广西高校科研资助项目〔桂教200103YB023〕

摘　　要：目的:探讨不同类型精子发生障碍人群中TEKT5基因的mRNA转录表达情况。方法:应用实时定量反转录聚合酶链式反应(QRT-PCR)的方法,分别以正常、弱精和少精症组cDNA为模板,扩增正常精子和异常精子中TEKT5基因在mRNA水平的差异。结果:正常组与少精组中TEKT5的表达量均显著高于弱精组(0.681 vs.0.316,P<0.05;0.527 vs.0.316,P<0.05),而正常组与少精组之间差异无统计学意义(0.681 vs.0.527,P>0.05)。结论:TEKT5基因表达是维持精子正常活力所必需的,男性精子TEKT5基因表达减少是精子活力低下的原因之一。Objective： To explore mRNA transcription and expression of TEKT5 gene in the population with different types of spermatogenesis failure. Methods： Real - time quantitative reverse transcription polymerase chain reaction （ qRT - PCR ） method was used to detect mRNA levels of TEKT5 gene in normal sperms and abnormal sperms with eDNA of normal cases, cases with asthenospermia, and eases with oligozoospermia. Results： The expression levels of TEKT5 in normal group and oligozoospermia group were statistically significantly higher than those in asthenospermia group （0. 681 vs. 0. 316 , P〈0. 05; 0. 527 vs. 0. 316, P〈0. 05） , but there was no statistically significant difference between normal group and oligozoospermia group （ 0. 681 vs. 0. 527, P 〉 0.05 ） . Conclusion ： Expression of TEKT5 gene is necessary to maintain normal vitality of sperms. Decrease of TEKT5 gene expression in male sperms is one of the causes of low sperm motility.

关 键 词：TEKT5 精子发生障碍 MRNA 

分 类 号：R321.1[医药卫生—人体解剖和组织胚胎学]